We narrowed to 9,287 results for: Pol;

1. pIS1 DICER1 short UTR

   Plasmid

   #21648

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Dicer 1 (DICER1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Expression

   Bacterial

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBW-m4046938

   Plasmid

   #145599

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCellFree_G03 CREBZF

   Plasmid

   #67070

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CREBZF (CREBZF Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. MET FLAG103QDProCFPp303

   Plasmid

   #15591

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 103Q Delta Pro (HTT Human)
   

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pGEM-myr-tdTomato-SV40pA-FKF

   Plasmid

   #119867

   Purpose

   Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   2x myristoylation

   Expression

   Bacterial

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p21 promoter D

   Plasmid

   #16465

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 promoter (CDKN1A Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCRW0.8 p21 (rat)

   Plasmid

   #16477

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 (Cdkn1a Rat)
   

   Expression

   Bacterial

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pIS1 Cyclin D2 long-mut-miR15/16

   Plasmid

   #21647

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Insert

   Cyclin D2 long-mut-miR15/16 (CCND2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutant miR15/16 microRNA binding sites

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. psicheck-2-Mut mod

    Plasmid

    #48165

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Mut (Mut Mouse)
    

    Use

    Luciferase

    Mutation

    A/T mutation in the ATG of the uORF

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pBP-ORF

    Plasmid

    #72949

    Purpose

    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - ORFs
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCellFree_G03 CSNK2A1

    Plasmid

    #67085

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMpGWB106-OEP7(1–50)

    Plasmid

    #100602

    Purpose

    Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

    Depositor

    Yutaka Kodama

    Article

    Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

    Insert

    OEP7(1–50)-Citrine (OEP7 Mustard Weed)
    

    Expression

    Plant

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p21 promoter F

    Plasmid

    #16467

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

15. GAL 25Q+ProCFPp303

    Plasmid

    #15574

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt25Q + Pro (HTT Human)
    

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pDEST15-Nedd4.2F823A

    Plasmid

    #165104

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d F823A

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

    Insert

    Nedd4.2d F823A (NEDD4L Human)
    

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    F823A; mutation for destabilization of trimer fo…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GAL HA25QDProCFPp303

    Plasmid

    #15578

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Tags

    CFP and HA

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pOPINK-TRABID (NZF1-3, aa 1-178)

    Plasmid

    #66185

    Purpose

    Expresses TRABID NZF1-3 (aa 1-178) in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    TRABID (ZRANB1 Human)
    

    Tags

    His6-GST

    Expression

    Bacterial

    Mutation

    only codes for amino acids 1-178 of TRABID

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. GAL HA103QDProCFPp303

    Plasmid

    #15579

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Tags

    CFP and HA

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pET15b-GB

    Plasmid

    #129689

    Purpose

    express E. coli GreB

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    his6/thrombin/GreB (greB E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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