Skip to main content
Addgene
Showing: 501 - 520 of 1036 results

  4. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

    Enlarge

  8. FSF-GFP

    Plasmid
    #163658
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR, TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TFR…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163658-plasmid-map-sequence-id-322789

    Enlarge

  9. FSS-GFP

    Plasmid
    #163654
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TFR…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163654-plasmid-map-sequence-id-322771

    Enlarge

  10. SSF-GFP

    Plasmid
    #163656
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163656-plasmid-map-sequence-id-322782

    Enlarge

  11. FFS-GFP

    Plasmid
    #163659
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TFR…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163659-plasmid-map-sequence-id-322790

    Enlarge

  12. SFS-GFP

    Plasmid
    #163655
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TFR and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163655-plasmid-map-sequence-id-322781

    Enlarge

  13. SFF-GFP

    Plasmid
    #163657
    Purpose
    Expresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and transferrin receptor (ST6GAL1, TFRC Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163657-plasmid-map-sequence-id-322784

    Enlarge

  14. ss-ED-mCherry-GPI

    Plasmid
    #163680
    Purpose
    Exprsses the GPI-anchored luminal domain of ST6GAL1 with a mCherry tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    The luminal domain of ST6 beta-galactoside alpha-2,6-sialyltransferase 1 with N-terminally tagged signal sequences (ST6GAL1 Human)
    Use
    Tags
    GPI anchor, mCherry, and signal sequences
    Expression
    Mammalian
    Mutation
    This chimera contains the ecto-domain of ST. Its …
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163680-plasmid-map-sequence-id-322820

    Enlarge

  15. GFP(N-glyc)-Tac-TC

    Plasmid
    #162496
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162496-plasmid-map-sequence-id-323476

    Enlarge

  16. TFORF3356

    Plasmid
    #144832
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NRF1 (NRF1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    144832-plasmid-map-sequence-id-471176

    Enlarge

  17. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid
    #224474
    Purpose
    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    EFS promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    224474-plasmid-map-sequence-id-447208

    Enlarge

  18. CLC-EGFP206(5)

    Plasmid
    #193573
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 5 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193573-plasmid-map-sequence-id-380171

    Enlarge

  19. CLC-EGFP206(6)

    Plasmid
    #193574
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 6 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193574-plasmid-map-sequence-id-380173

    Enlarge

  20. CLC-EGFP206(7)

    Plasmid
    #193575
    Purpose
    Expresses human Clathrin Light Chain B tagged with mEGFP at amino acid position 206 with a rigid alpha helix of 7 amino acids in mammalian cells.
    Depositor
    Justin Taraska
    Article
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Insert
    Clathrin light chain B (CLTB Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 207-211
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193575-plasmid-map-sequence-id-383667

    Enlarge

Showing: 501 - 520 of 1036 results