We narrowed to 34,102 results for: Eng
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Plasmid#8827DepositorInsertTEV protease, S219V mutant
TagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)Available SinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-pT231 scFv-GFP
Plasmid#218827PurposeYeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFPDepositorInsertpT231 tau scFv
ExpressionYeastPromoterGAL1-10Available SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHB-4
Plasmid#140957PurposeFor PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Prha-base
Plasmid#169244PurposeSuicide vector for integrating expression cassette in P. putida (L-rhamnose induction)DepositorInsertrhaRS
ExpressionBacterialPromoterrhaSAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS-APTA
Plasmid#247077PurposepCS27 containing aroL, ppsA, tktA, aroG(fbr) from E. coliDepositorInsertsaroL
ppsA
tktA
aroG(fbr)
ExpressionBacterialMutationfeedback-inhibition-resistantPromoterPLlacO1Available SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK792
Plasmid#8830DepositorInsertTEV protease, S219P mutant
TagsHis, MBP (cleaved), and polyarginineExpressionBacterialMutationS219P mutationAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
ARK2aa
Plasmid#81013PurposeE.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-aphA
and PMD
PromoterLacUV5 and Trc promoterAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BYG
Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Actinin-sstFRET-GR
Plasmid#83416PurposeFRET-based actinin tension sensorDepositorInsertActinin-sstFRET-GR
ExpressionMammalianPromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyExpressionBacterialMutationL401GPromoterPalkSAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP11
Plasmid#215559PurposepKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 originDepositorInsertsCas9
gRNA targeting pBR322 origin of replication
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23105 and tetAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-T7Max-UTR1-deGFP-8xHis-T500
Plasmid#178422PurposeExpression of deGFP via a T7 Promoter in TXTLDepositorInsertdeGFP
UseSynthetic BiologyTags8xHis TagExpressionBacterialPromoterT7MaxAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHB-3
Plasmid#140956PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.DepositorInsertphaCAB
ExpressionBacterialAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
ExpressionBacterialAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
KO plasmid
Plasmid#135970PurposeExpresses SpCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only