We narrowed to 32,119 results for: Eng;

1. pCas2C

   Plasmid

   #82374

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

   Plasmid

   #169586

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ARK2aa

   Plasmid

   #81013

   Purpose

   E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)

   Depositor

   Taek Soon Lee

   Article

   Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

   Inserts

   MevTo-BBa1002-pTrc-Mkco-aphA
   and PMD
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   LacUV5 and Trc promoter

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. ptrc99a-pVhb-tdTER-fdh

   Plasmid

   #215649

   Purpose

   pBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   trans-enoyl-CoA reductase
   formate dehydrogenase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Vhb

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRC3EGFP

   Plasmid

   #193790

   Purpose

   Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PR promoter

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVH231-Tier1-PmPGK1-mTagBFP2

   Plasmid

   #169520

   Purpose

   Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVH607-Tier1-PCREm4-Nluc

   Plasmid

   #169594

   Purpose

   Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PCREm4

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH233-Tier1-PmPGK1-eYFP

   Plasmid

   #169522

   Purpose

   Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven enhanced yellow fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTCW012

   Plasmid

   #83527

   Purpose

   Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pFUS1J2

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-T7Max-UTR1-deGFP-8xHis-T500

    Plasmid

    #178422

    Purpose

    Expression of deGFP via a T7 Promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    8xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCORE-Kp53

    Plasmid

    #72241

    Purpose

    Contains the CORE cassette with KanMX4 and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    kanMX4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBTRCk-pVhb-maACR

    Plasmid

    #215651

    Purpose

    pBBR1 Origin, KanR, with maACR gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    acyl-CoA reductase
    

    Use

    Tags

    Maltose binding protein and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYC-pVhb-vfFadBA

    Plasmid

    #215650

    Purpose

    pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Vhb

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY2

    Plasmid

    #218115

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY3

    Plasmid

    #218116

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY3-LYS-IV

    Plasmid

    #218119

    Purpose

    LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNVLTv2-pvdIJD-Ptrc-base

    Plasmid

    #169243

    Purpose

    Suicide vector for integrating expression cassette in P. putida (IPTG induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-Casp2

    Plasmid

    #50023

    Purpose

    pENTR vector for mouse Caspase-2

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    Casp2 (Casp2 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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