We narrowed to 8,693 results for: PAN

1. pYPQ146-ZmUbi-tRNA

   Plasmid

   #158404

   Purpose

   Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Maize ubiquitin 1

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ135-tRNA2.0

   Plasmid

   #158397

   Purpose

   Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Without promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ136B2.0

   Plasmid

   #167160

   Purpose

   Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU3

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ136-tRNA2.0

   Plasmid

   #158398

   Purpose

   Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Without promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ145-ZmUbi-tRNA

   Plasmid

   #158403

   Purpose

   Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Maize ubiquitin 1

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ141-ZmUbi-RZ-Aac.4

   Plasmid

   #158406

   Purpose

   Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Maize ubiquitin 1

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ141-ZmUbi-RZ-Aa3.8.5

   Plasmid

   #158407

   Purpose

   Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Maize ubiquitin 1

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cultivarium MACKEREL Library

   Pooled Library

   #208054

   Purpose

   pGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2024.02.23.581749

   Use

   Synthetic Biology

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-TetOne-Puro-LUC

    Plasmid

    #218630

    Purpose

    This plasmid contains luciferase under doxycycline inducible control.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    LUC (LOC116160065 )
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEVOL-ABK

    Plasmid

    #126035

    Purpose

    Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:

    Inserts

    tRNA synthetase 1
    tRNA synthetase 2
    tRNA for TAG codon
    

    Expression

    Bacterial

    Mutation

    L274M, C313A, Y349F

    Promoter

    araBAD promoter, glnS promoter, and proK promoter

    Available Since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ-dpcoCas9-SunTag

    Plasmid

    #158410

    Purpose

    CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBMN-AS-YY1

    Plasmid

    #154943

    Purpose

    Knocking down of human YY1 through shRNA and amiRNA targeting YY1

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    shRNA and amiRNA against YY1 (YY1 Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM4788

    Plasmid

    #120089

    Purpose

    Cyanobacteria synthetic plasmid harboring the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS) to test the pSYSA-TA1 toxin–antitoxin cassette.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

    Plasmid

    #182359

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Ultra-pan-neuronal positive control pUNP::gfp(NLS)
    

    Expression

    Worm

    Promoter

    Promoter Ultra Pan Neuronal

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. C-term png in pGEX4

    Plasmid

    #112975

    Purpose

    For protein expression and purification of the C-term of Drosophila png

    Depositor

    Terry Orr-Weaver

    Article

    Fenger et al Development. 2000 Nov;127(22):4763-74.

    Insert

    png (png Fly)
    

    Expression

    Bacterial

    Mutation

    wild-type C terminus

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. C2 png genomic in Casper

    Plasmid

    #112971

    Purpose

    P element vector for transposon insertion of png genomic DNA into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Fenger et al Development. 2000 Nov;127(22):4763-74.

    Insert

    png genomic DNA (png Fly)
    

    Use

    P insertion vector

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. C2 M2 png-myc in Casper4

    Plasmid

    #112968

    Purpose

    P element vector for transposon insertion of Myc-tagged png into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Fenger et al Development. 2000 Nov;127(22):4763-74.

    Insert

    png-myc (png Fly)
    

    Use

    P insertion vector

    Tags

    myc epitope

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87828

    Purpose

    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ-Cas9-Act3.0

    Plasmid

    #178954

    Purpose

    It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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