We narrowed to 25,019 results for: lars;

1. pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP

   Plasmid

   #156448

   Purpose

   For transient expression of human CTCF tagged with eGFP

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   human CTCF (CTCF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Is3xP3-mCherry

   Plasmid

   #220091

   Purpose

   Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)

   Depositor

   Monika Gulia-Nuss

   Article

   Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

   Insert

   3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Is3xP3

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-EN1680_Sororin-Cterm

   Plasmid

   #156451

   Purpose

   For transient expression of Cas9 and sgRNA targeting mouse Cdc5a (SORORIN) stop codon

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   Cas9, SORORIN (Cdca5 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcrDNA3.1-PRKACA

   Plasmid

   #100890

   Purpose

   Mammalian expression of PRKACA

   Depositor

   Sanford Simon

   Article

   Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

   Insert

   Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Isoform 1 wild type

   Promoter

   CMV

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+-N-PAS2-GAF-PHYB-MCherry-CAAX

   Plasmid

   #154910

   Purpose

   1-621 amino acids of Arabidopsis PHYB (Gene ID: 816394), tagged with mCherry fluorescence protein and the CAAX membrane moiety. Mammalian codon optimised.

   Depositor

   Jonathan Clarke, Clare Buckley

   Article

   Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

   Insert

   Phytochrome B (PHYB Mustard Weed, Synthetic)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   CAAX membrane moiety and mCherry

   Expression

   Mammalian

   Mutation

   1-621 amino acids of Arabidopsis PHYB

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+-Y276H PHYB-CAAX

   Plasmid

   #154911

   Purpose

   1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

   Depositor

   Jonathan Clarke, Clare Buckley

   Article

   Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   CAAX membrane moiety and mCherry

   Expression

   Mammalian

   Mutation

   1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNoROS-CAT

   Plasmid

   #170152

   Purpose

   Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation

   Depositor

   Dimitri Pestov

   Article

   Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.

   Inserts

   catalase (Cat Mouse)
   roGFP2
   

   Use

   Genome integration via sleeping beauty transposon…

   Tags

   FLAG tag and Nucleolar Localization Sequence (NoL…

   Expression

   Mammalian

   Mutation

   Promoter

   RPBSA and TRE promoter

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

   Plasmid

   #156431

   Purpose

   Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   RAD21, Halotag (Rad21 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

   Plasmid

   #156433

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   SORORIN, AID (Cdca5 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156434

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    All zinc fingers mutated

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156430

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Cterm_Nterm_switched

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid

    #156429

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    DELTA1-265

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SV40 1: pBSSVD2005

    Plasmid

    #21826

    Depositor

    David Ron

    Insert

    SV40 Large T (SV40gp6 simian virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    likely expresses aa109-708 of the large T antigen

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #156432

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKC-Tol2

    Plasmid

    #85600

    Purpose

    Source of Tol2 transposase driven by a mini-CAGS promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mini-CAG

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKUb-Tol2

    Plasmid

    #85601

    Purpose

    Source of Tol2 transposase driven by a human ubiquitin promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Ubiquitin

    Available since

    Feb. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKTol2C-EGFP

    Plasmid

    #85598

    Purpose

    Tol2 transposon with EGFP driven by minimal-CAGs promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposon with EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mini-CAG

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GGE0029

    Plasmid

    #120730

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKTol2P-PTK

    Plasmid

    #85599

    Purpose

    Tol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Tol2 transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPTn2P-PTK

    Plasmid

    #85604

    Purpose

    Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

    Depositor

    Karl Clark, Scott Fahrenkrug

    Article

    Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

    Insert

    Passport transposon with a puromycin-thymidine kinase fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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