We narrowed to 1,548 results for: tetracycline resistance

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

Plasmid

#176836

Purpose

Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP

Use

Unspecified

Mutation

See Depositor Comments Below

Promoter

Tet operator (tetracycline inducible expression)

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin

Plasmid

#166603

Purpose

Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Promoter

tetracycline response element

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Klentaq

Plasmid

#157862

Purpose

Plasmid for the aTc-inducible expression of the Klentaq

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

Klentaq

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP-T847E

Plasmid

#71110

Purpose

mammalian expession of Flag-CtlP T847E mutation

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T847E

Promoter

TRE promoter, Tet ON

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRS2 (CK2alpha', CK2beta)

Plasmid

#27093

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha' (CSNK2A2 Human)

Use

Tetracycline-regulated expression

Tags

HA and Myc

Expression

Mammalian

Promoter

bidirectional tet-responsive promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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tet H-ras val12

Plasmid

#8900

Depositor

Lynda Chin

Article

Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

Insert

ras val12 (HRAS Human)

Tags

Tet-op promoter

Expression

Mammalian

Mutation

G12V

Available Since

Jan. 13, 2006

Availability

Academic Institutions and Nonprofits only
pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

Plasmid

#236251

Purpose

Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

tTS/rtTA
cymR

Use

Lentiviral

Expression

Mammalian

Mutation

rTS and rtTA_M2 linked by T2A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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q4wt

Plasmid

#24502

Depositor

Bruce Conklin

Article

Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Insert

Gq alpha

Tags

HA (see comments)

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only
integrin beta6 pcDNA1 neo

Plasmid

#13580

Depositor

Dean Sheppard

Article

Weinacker et al J Biol Chem. 1994 Mar 4. 269(9):6940-8.

Insert

integrin beta 6 (ITGB6 Human)

Expression

Mammalian

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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Human ICAM-2

Plasmid

#8634

Depositor

Timothy Springer

Article

Staunton et al Nature 1989 May 4;339(6219):61-4.

Insert

ICAM-2 (ICAM2 Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-hM3Dq-mCherry

Plasmid

#166599

Purpose

Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

Plasmid

#86194

Purpose

beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

Depositor

Robert Singer

Article

Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

Insert

beta Globin (HBB, Hbb Mouse, Human)

Use

Tet on

Expression

Mammalian

Mutation

39TER in beta Globin in MCS II

Promoter

Tet on

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_RNAseH1

Plasmid

#65783

Purpose

constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

Depositor

Thomas Tuschl

Insert

RNaseH1 (RNASEH1 Zebrafish, Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

Plasmid

#154088

Purpose

Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

Plasmid

#87828

Purpose

High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Article

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGV13 (CK2alpha K68M, CK2beta)

Plasmid

#27094

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Inserts

CK2beta (CSNK2B Human)
CK2alpha K68M (CSNK2A1 Human)

Use

Tetracycline-regulated expression

Tags

HA on CK2alpha and Myc on CK2beta

Expression

Mammalian

Mutation

K68M on CK2alpha

Promoter

bidirectional tet-responsive promoter and bidirec…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

Plasmid

#154089

Purpose

Improved version to express RUNX1, P2A, ERG in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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