We narrowed to 10,059 results for: Uty

1. pDYT002

   Plasmid

   #186550

   Purpose

   Lineage tracing vector (with barcoded Target Sites and triple sgRNAs

   Depositor

   Jonathan Weissman

   Article

   Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

   Insert

   14-bp random integration barcode and three target sites
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. His-FLAG-Tev_ACTA1 (alpha-actin)

   Plasmid

   #188452

   Purpose

   Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

   Depositor

   Roberto Dominguez

   Article

   Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

   Insert

   ACTA1 (ACTA1 Human)
   

   Tags

   6xHis-FLAG

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PL-5LTR-GW-A

   Plasmid

   #138480

   Purpose

   For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEMS2113

   Plasmid

   #49138

   Purpose

   AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   ssAAV-Ple67-emGFP-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   FEV

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCEP4-HA-Flag-natT1R2 ECD-MHC

   Plasmid

   #113957

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG, Signal/leader sequence from influenza hemag…

   Expression

   Mammalian

   Mutation

   residues 22-568 fused to a transmembrane tether

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-A3A(N57Q)-BE3

   Plasmid

   #158157

   Purpose

   T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

   Depositor

   Keith Joung

   Article

   Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

   Insert

   6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
   

   Tags

   6xHis tag on N-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Nsp7-EGFP

   Plasmid

   #165111

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp7 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMH-SFB-USP7

   Plasmid

   #99393

   Purpose

   Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   USP7 (USP7 Human)
   

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJM185A

   Plasmid

   #218126

   Purpose

   Hybrid circuit displaying hsCRBN bait and driving expression of luxAB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   CRBN (CRBN Human)
   luxAB
   

   Tags

   434cI_RR69

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

    Plasmid

    #183352

    Purpose

    Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    TVA, EGFP and tTA
    

    Use

    AAV

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA5-TEX264-deltaLRR-cSSH

    Plasmid

    #220210

    Purpose

    Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    c.4-75del / p.2-24del

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTarget: Ptaq-RFP_PlacIq-GFP

    Plasmid

    #192280

    Purpose

    RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Inserts

    RFP
    GFP
    

    Expression

    Bacterial

    Promoter

    PlacIq and Ptaq

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN-CAG-mRFP-PCNA pc2729

    Plasmid

    #166040

    Purpose

    Expresses mRFP tagged human PCNA in mammalian cells.

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEMS-Tom20-mEGFP-reHaloTagS

    Plasmid

    #200189

    Purpose

    Tom20 with reversible HaloTagS variant for fluorescent labeling

    Depositor

    Jacob Piehler

    Article

    Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

    Insert

    Tom20 (TOMM20 Human)
    

    Tags

    mEGFP and reHaloTagS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAdCMV/V5-DEST-STAT3C-3xFlag

    Plasmid

    #99264

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Mutation

    A662C N664C

    Promoter

    CMV

    Available Since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEMS1991

    Plasmid

    #49122

    Purpose

    AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple67-iCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    FEV

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Nsp14-mCherry

    Plasmid

    #165137

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp14 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOGS539_GLuc-ns

    Plasmid

    #226719

    Purpose

    Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Gaussia Luciferase
    

    Tags

    HA tag

    Expression

    Yeast

    Promoter

    pTEF1

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-TagRFP658-P2A-GFP

    Plasmid

    #178971

    Purpose

    Expresses TagRFP658 and GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658-P2A-GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBT9-Hook3

    Plasmid

    #198525

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook3 (HOOK3 Human)
    

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge