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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 39,543 results for: ina

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Showing: 5181 - 5200 of 39543 results

  3. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

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  6. M3-M14-dCas9

    Plasmid
    #134779
    Purpose
    Expression of fusion protein M3-M14-dCas9 in Mammalian cells
    Depositor
    Shu-Bing Qian
    Publication
    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 S. pyogenes, Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134779-plasmid-map-sequence-id-267134

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  7. pcDNA3-N-Flag-NLRP3 91-710

    Plasmid
    #75140
    Purpose
    Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    fragment encoding amino acids 91-710
    Promoter
    CMV
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75140-plasmid-map-sequence-id-142337

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  9. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50589-plasmid-map-sequence-id-75662

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Showing: 5181 - 5200 of 39543 results