We narrowed to 11,028 results for: HA

1. 6xHis(TEV)-yDrp1-StrepII

   Plasmid

   #174427

   Purpose

   Expresses S. pombe Drp1 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-wbp2nlT45A

   Plasmid

   #172464

   Purpose

   expresses protein with one aa substitution to affect potential phosphorylation

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlT45A
   

   Use

   Xenopus expression

   Tags

   none

   Mutation

   changed Threonine at aa45 to Alanine

   Promoter

   SP6

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBlastR-GMRWhite

   Plasmid

   #165885

   Purpose

   Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Transgenesis Ready Selection Vector
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31

   Plasmid

   #165888

   Purpose

   Puromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   All-in-one FC31 RMCE Cassette
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31

   Plasmid

   #165889

   Purpose

   Blasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   All-in-one FC31 RMCE Cassette
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGal4BD 1-147

   Plasmid

   #165819

   Purpose

   1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Gal4BD 1-147
   

   Expression

   Insect

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAL4AD 768-881

   Plasmid

   #165820

   Purpose

   768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   GAL4AD 768-881
   

   Expression

   Insect

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM80

   Plasmid

   #173173

   Purpose

   HA-dCas9

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWSK29-wzzBST

   Plasmid

   #172968

   Purpose

   pWSK29 with Salmonella typhimurium wzzBST gene

   Depositor

   Renato Morona

   Article

   Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

   Insert

   wzzB gene from Salmonella enterica serovar Typhimurium
   

   Expression

   Bacterial

   Mutation

   K198E- please see depositor comments

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWSK29-fepEST

    Plasmid

    #172969

    Purpose

    pWSK29 with Salmonella typhimurium fepEST gene

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Insert

    fepE gene from Salmonella enterica serovar Typhimurium
    

    Expression

    Bacterial

    Mutation

    G160S- please see depositor comments

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. C514-E59: attB5r-RU5u-2xHA-nostop-attB1r

    Plasmid

    #162966

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Dual HA epitope tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGMR-WhiteA2

    Plasmid

    #165874

    Purpose

    Basic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Eye Marker Cassette
    

    Use

    Synthetic Biology

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPuroR-GMRWhite

    Plasmid

    #165884

    Purpose

    Puromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

    Plasmid

    #162805

    Purpose

    STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    Ub-BsmBI-HA_IRES_Blasticidin
    

    Use

    Piggybac

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWDY1226

    Plasmid

    #138593

    Purpose

    Expresses NatL2-R156A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Arginine 156 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWDY1222

    Plasmid

    #138592

    Purpose

    Expresses NatL2-K418A in E.coli

    Depositor

    James Naismith

    Article

    Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

    Insert

    phenylacetate-coenzyme A ligase
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    changed Lysine 418 to Alanine

    Promoter

    T7

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMET17p-CCG 316

    Plasmid

    #131166

    Purpose

    For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

    Type

    Empty backbone

    Tags

    CCG

    Expression

    Yeast

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS_KS_attB2_SA(2)_T2AGAL80

    Plasmid

    #125219

    Purpose

    Artificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    GAL80
    

    Use

    Other

    Tags

    T2A

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

    Plasmid

    #91945

    Purpose

    Bacterial expression of maize SAE1 and truncated SAE2

    Depositor

    Richard Vierstra

    Article

    Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

    Inserts

    HA-ZmSAE1
    ZmSAE2-FLAG
    

    Tags

    FLAG and HA

    Expression

    Bacterial

    Mutation

    Truncation of ZmSAE2 to mimic a splice variant

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNCMV HPV8 E6 R138S

    Plasmid

    #87659

    Purpose

    Flag/HA tagged HPV8 E6 R138S

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    R138S

    Promoter

    CMV

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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