We narrowed to 15,981 results for: LEA

1. p2Flag CMV2-ZO-2-1st-PDZ-mut

   Plasmid

   #27416

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   K38E, F44H, I46E and V48E

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

2. PcDNA-DEST53-CypB-Nucl N-term GFP

   Plasmid

   #36123

   Depositor

   Patrick Van Oostveldt

   Article

   Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

   Insert

   cyclophilin B (PPIB Human, contains CypB AA 33-216)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   A212S

   Promoter

   CMV

   Available Since

   Nov. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pOCC215

   Plasmid

   #118879

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
   

   Tags

   Dendra2, HIS6, cleavable with 3C protease

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry_LD 0_CS M_tTA-BFP

   Plasmid

   #58874

   Purpose

   MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

   Plasmid

   #52547

   Purpose

   Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attP (full length)
   Neo
   PhiC31o integrase
   

   Expression

   Mammalian

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry_LD 0_CS A_tTA-BFP

   Plasmid

   #58873

   Purpose

   MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC207

   Plasmid

   #118869

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherry

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease, mCherry

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBER

   Plasmid

   #62662

   Purpose

   Donor vector for tdTomato knock-in via landing pad

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Inserts

   tdTomato
   hygromycin
   

   Use

   Cre/Lox

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBGX

   Plasmid

   #62666

   Purpose

   Donor vector for Dre knock-in via landing pad

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   Dre
   

   Use

   Cre/Lox

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWay21-MKP-1CH2AB

    Plasmid

    #13475

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1CH2AB (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Contains aa 1-136

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pIS1 IMP-1 short UTR

    Plasmid

    #21639

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLaNGo11-3

    Plasmid

    #126054

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMiNGo11-1

    Plasmid

    #126056

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    mitochondria Rieske iron sulphur protein (1-100 aa)
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rae1-pDONR221

    Plasmid

    #124240

    Purpose

    Gateway entry clone of Drosophila Rae1 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Rae1 (Rae1 Fly)
    

    Expression

    Insect

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGex2t SadBs

    Plasmid

    #66882

    Purpose

    Bacterial expression vector encoding N-terminally GST-tagged short isoform of human SadB

    Depositor

    Maria Alvarado-Kristensson

    Article

    Eklund et al J Biol Chem. 2014 Aug 1;289(31):21360-73

    Insert

    SadB (BRSK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

16. GFP-ERK2 del174-177

    Plasmid

    #118297

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 del174-177 (Mapk1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion 174aa-177aa

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC110

    Plasmid

    #118905

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and LPETGg

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC219

    Plasmid

    #118908

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
    

    Tags

    YBBR tag, HIS10, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

    Plasmid

    #52540

    Purpose

    Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Expression

    Mammalian

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNB695

    Plasmid

    #60144

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Promoter

    MET17pr

    Available Since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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