We narrowed to 13,665 results for: crispr cas9

1. pAAV-U6-sgRNA-CMV-GFP

   Plasmid

   #85451

   Purpose

   Express sgRNA in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pCMV-EGFP
   

   Use

   AAV

   Promoter

   pCMV-EGFP

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ132B

   Plasmid

   #69282

   Purpose

   Golden Gate entry vector; 2nd gRNA under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Promoter

   Promoterless

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM178

   Plasmid

   #61270

   Purpose

   Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   pLtetO, native, native (respectively)

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT7

   Plasmid

   #204292

   Purpose

   For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-DsRed
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF278

   Plasmid

   #167673

   Purpose

   Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSGAb-km

   Plasmid

   #121999

   Purpose

   A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Insert

   none
   

   Use

   CRISPR

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBEclos-cbei2385-g2-opt

   Plasmid

   #118215

   Purpose

   CRISPR-mediated base editing in Clostridium

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol Bioeng. 2019 Feb 10. doi: 10.

   Insert

   Apobec1, Casp nickase, UG1-sgRNA
   

   Expression

   Bacterial

   Promoter

   Ptm

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSGAb-spe

   Plasmid

   #122000

   Purpose

   A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Insert

   none
   

   Use

   CRISPR

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDN-Cherry-sgRNA

    Plasmid

    #221387

    Purpose

    episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs

    Depositor

    Deborah Hung

    Article

    Neo et al J Bacteriol. 2024 Mar 21;206(3):e0033523

    Insert

    none, cloning vector for introduction of targeting sequences
    

    Use

    CRISPR

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT71

    Plasmid

    #204305

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ131B

    Plasmid

    #69281

    Purpose

    Golden Gate entry vector; 1st gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF464

    Plasmid

    #167672

    Purpose

    Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF463

    Plasmid

    #167671

    Purpose

    Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ131C

    Plasmid

    #69284

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ132C

    Plasmid

    #69285

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORE303N

    Plasmid

    #194438

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, KpnI site for gRNA insertion

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ132A

    Plasmid

    #69274

    Purpose

    Golden Gate entry vector; 2nd gRNA under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ133B

    Plasmid

    #69283

    Purpose

    Golden Gate entry vector; 3rd gRNA under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJAT31

    Plasmid

    #204291

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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