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pEMS2021

Plasmid

#49128

Purpose

AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple302-iCre

Use

AAV

Expression

Mammalian

Promoter

DCX

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1997

Plasmid

#49127

Purpose

AAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple198-iCre

Use

AAV

Expression

Mammalian

Promoter

SLC6A4

Available Since

May 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1-Puro CAG-CLTX CD32a ITAM CAR

Plasmid

#171964

Purpose

Donor plasmid for CLTX CD32a-ITAM CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a ITAM CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS-EGFP^PTC-231

Plasmid

#254989

Purpose

Express EGFP^PTC-231 in mammalian cells

Depositor

Joshua Mendell

Article

Zhu et al Cell Rep. 2020 Jul 14;32(2):107895. doi:

Insert

EGFP^PTC-231

Expression

Mammalian

Mutation

NA

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-NDi-H1.2_WT-Halo

Plasmid

#247484

Purpose

Expresses H1.2 (WT) by tet-on system

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (HIST1H1C) (H1-2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

TRE3G

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS-NDi-H1.2_Khalf-Halo

Plasmid

#247485

Purpose

Expresses H1.2 (Khalf) by tet-on system

Depositor

Kazuhiro Maeshima

Article

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.2 (H1-2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

Half of Lysine in C-terminal IDR are replaced by …

Promoter

Tet-responsive element

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1 locus donor plasmid

Plasmid

#252870

Purpose

Plasmid encoding FRT recombination site and TetOn3G transactivator flanked by AAVS1 homology arms

Depositor

Norman Davey

Insert

TetOn3G transactivator and FRT recombination site

Expression

Mammalian

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1 gRNAs-Cas9D10A-mRuby

Plasmid

#252799

Purpose

Plasmid for paired targeting of AAVS1 locus via Cas9(D10A)nickase

Depositor

Norman Davey

Insert

Cas9(D10A)

Tags

mRuby

Expression

Mammalian

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-6XPyltRNA-dCas9K1151TAG-IRESPylRS

Plasmid

#248695

Purpose

Expression dead CRISPR/Cas9 with unnatural amino acid incorporated at K1151 residue

Depositor

Shuibing Chen

Article

Zhu et al Proc Natl Acad Sci U S A. 2025 Oct 7;122

Insert

dead CRISPR/Cas9 K1151TAG, MbPylRS

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pYJ41-AAVS1-XLone-Mafa

Plasmid

#131084

Purpose

Donor plasmid of targeted inducible-Mafa knockin

Depositor

Xiaojun Lian

Insert

MAFA (MAFA Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pYJ27-AAVS1-XLone-NKX6.1

Plasmid

#131070

Purpose

Donor plasmid of targeted inducible-NKX6.1 knockin

Depositor

Xiaojun Lian

Insert

NKX6.1 (NKX6-1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Blast-PECAM1-Neo

Plasmid

#221149

Purpose

Expression of platelet and endothelial cell adhesion molecule 1 (CD31)

Depositor

Paul Burridge

Article

Pinheiro et al Sci Rep. 2024 Mar 26;14(1):7123. doi: 10

Insert

Platelet And Endothelial Cell Adhesion Molecule 1 (PECAM1 Human)

Expression

Mammalian

Promoter

PECAM1

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactive+sgAAVS1_145

Plasmid

#213170

Purpose

Vector with sgAAVS1 used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgAAVS1_145

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-TurboID-HA-rtTA

Plasmid

#226995

Purpose

Backbone plasmid to express TurboID fusion proteins in mammalian cells

Depositor

Bruno Dallagiovanna

Article

Gomes-Junior et al PLoS One. 2025 May 30;20(5):e0324779. do

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Tags

SNAP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Blast

Plasmid

#214014

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Hygro

Plasmid

#214015

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Blast-TNNT2-Zeo

Plasmid

#214018

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODALvar-matMYC

Plasmid

#115641

Purpose

For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

Plasmid

#179435

Purpose

Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-coreHS4-mch (SC)

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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MKC89_CCR5(UbC-tEPOR-2A-YFP)

Plasmid

#232404

Purpose

AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.

Depositor

Kyle Cromer, Matthew Porteus

Article

Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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JD179

Plasmid

#229496

Purpose

Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes and constitutive VA RNA I gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, L4-22k/33k, VA RNA I

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Tre (E2A, L4-22k/33k). Wt promoter (VA RNA I), ET…

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFB_ROSA26_SpCas9_gRNAs

Plasmid

#174703

Purpose

Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

Depositor

Beverly L. Davidson

Article

Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

Insert

SpCas9 dual gRNAs

Use

AAV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAC-mCherry2-G4

Plasmid

#164167

Purpose

Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAT-mCherry2-G

Plasmid

#164169

Purpose

Expresses mCherry2 under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Use

AAV

Expression

Mammalian

Promoter

tetO

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2214

Plasmid

#158417

Purpose

AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple265 (PCP2 Human)

Use

AAV

Promoter

Ple265

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn-FAS-DO-ReaChR-mCitrine-WPRE

Plasmid

#64035

Purpose

Expresses ReaChR under hSyn promoter. FAS-DO turns off expression in Cre-positive cells. FAS is compatible with other traditional Flex/DIO constructs

Depositor

Karel Svoboda

Insert

hSyn-FAS-DO-ReaChR-mCitrine-WPRE

Use

AAV

Tags

ReaChR tagged with mCitrine

Expression

Mammalian

Promoter

hSyn

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1991

Plasmid

#49122

Purpose

AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

ssAAV-Ple67-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

FEV

Available Since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP

Plasmid

#207862

Purpose

AAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AV0.1-BWS-copGFP

Plasmid

#124967

Purpose

Express copGFP under EF1 promoter

Depositor

Kazuhiro Oka

Insert

copGFP

Use

AAV

Promoter

EF1-alpha

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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