We narrowed to 32,620 results for: FER
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Plasmid#218018PurposeGST-tag fused to TEV Protease cleavage site for protein purification and cleavageDepositorInsertGST-tag
UseSynthetic BiologyTagsTEV protease cleavage siteAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-848
Plasmid#218003PurposeGST-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsertGST-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7-S72A
Plasmid#221556PurposeExpresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertRab7A (RAB7A Human)
UsePiggybacTagsmCherryExpressionMammalianMutationChange serine 72 to alaninePromoterEF1aAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)
Plasmid#221552PurposeExpresses Cas9 and gRNA for disruption of Rab7A gene in human cellsDepositorAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212830PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
TagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianPromoterCAG, TetOnAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212831PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
TagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianPromoterCAG, TetOnAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-PPM1H S124A/S211A
Plasmid#208370PurposeMammalian expression of HA-tagged human PPM1H S124A/S211ADepositorAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 myc-Rab10-mito
Plasmid#208367PurposeMammalian expression of myc-tagged human Rab10 with C-terminal mito signalDepositorAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)
Plasmid#208974PurposeRecruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)
Plasmid#208971PurposeRecruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)
Plasmid#208970PurposeRecruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus R200W
Plasmid#209179PurposeMammalian expression of SPAX8-related missense mutation (R200W) in human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (R200W) fused with Venus (NKX6-2 Human)
TagsVenusExpressionMammalianMutationNKX6-2 (R200W)PromoterCMVAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2(1-196)-Venus
Plasmid#209182PurposeMammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.DepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus L163V
Plasmid#209178PurposeMammalian expression of SPAX8-related missense mutation (L163V) in human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (L163V) fused with Venus (NKX6-2 Human)
TagsVenusExpressionMammalianMutationNKX6-2 (L163V)PromoterCMVAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Δ37 PPM1H
Plasmid#207334PurposeBacterial expression of His SUMO Δ37 PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-L2D/V9D/I19D PPM1H
Plasmid#207341PurposeMammalian expression of HA-L2D/V9D/I19D PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO Rab10(Q68L)
Plasmid#207342PurposeBacterial expression of His SUMO Rab10(Q68L)DepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-Δ115-133, 204-217 PPM1H
Plasmid#207343PurposeMammalian expression of HA-Δ115-133, 204-217 PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-B4GALT3mut
Plasmid#203602PurposeReporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.DepositorInsertbeta-1,4-galactosyltransferase 3 (B4GALT3 Human)
UseLuciferaseExpressionMammalianMutationSeed sequences in miR-27b target sequences were m…Available SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2(1-188)-Venus
Plasmid#203714PurposeSPAX8-related truncated mutation (E189*) containing the amino acids 1 to 188 of human NKX6-2 fused with the Venus fluorescent proteinDepositorInsertNKX6-2 (1-188) fused with Venus (NKX6-2 Human)
TagsVenusExpressionMammalianMutationdeletion of amino acids 189-277PromoterCMVAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only