We narrowed to 166,761 results for: Gene

1. pTRE-TAZ-CAMTA1

   Plasmid

   #235680

   Purpose

   Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

   Depositor

   Duojia Pan

   Article

   Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

   Insert

   TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCA.66/2272

   Plasmid

   #22733

   Depositor

   Mark Magnuson

   Article

   Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

   Insert

   Lox66-pU(delta)TK-EM7Neo-Lox2272
   

   Use

   Cre/Lox; Bac recombineering

   Mutation

   This vector was designed for assembling gene targ…

   Available Since

   Dec. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-hGRIN2B-CAG-ps-SpCas9

   Plasmid

   #102851

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

   Plasmid

   #64220

   Purpose

   Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64219

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHL-EF1a-SphcCas9-iP-A

   Plasmid

   #60599

   Purpose

   Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Insert

   CRISPR Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Codon usage optimized for human usage

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p426_Cas9_gRNA-R-RDS1a

   Plasmid

   #87405

   Purpose

   p426_Cas9_gRNA-RDS1a without the ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

   Plasmid

   #159676

   Purpose

   Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
   

   Expression

   Insect

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS

   Plasmid

   #140196

   Purpose

   Chromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   dSpCas9-VPR
   Bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   

   Use

   CRISPR and Synthetic Biology; Fungal genome integ…

   Tags

   VPR (VP64-p65-Rta) , NLS

   Promoter

   PgpdA and PtrpC

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. hum alpha ENAC promoter a25-a23

    Plasmid

    #83427

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

    Insert

    human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Promoter

    alpha ENAC

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-PHFF

    Plasmid

    #100280

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDN-D2irTNG4kwh

    Plasmid

    #44722

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    htetR::NLS::EGFP
    

    Use

    Synthetic Biology; Expression regulator/reporter;…

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9-Puro HLAG-2A-sgRNA

    Plasmid

    #182551

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2A-sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. LoxP2-Mb2Tomato-2A (SO240)

    Plasmid

    #99614

    Purpose

    To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LoxP1-His-H2B-Cherry-2A (SO290)

    Plasmid

    #99616

    Purpose

    To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    6xHis-H2B-Cherry
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pROSA26-Donor

    Plasmid

    #60952

    Purpose

    Porcine ROSA26 locus targeting

    Depositor

    Liangxue Lai

    Article

    Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

    Insert

    Porcine ROSA26 homologous ARM
    

    Use

    Gene targeting

    Tags

    Neo EGFP

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. LoxP2-H2B-EGFP-V5-2A (SO107)

    Plasmid

    #99617

    Purpose

    To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    H2B-EGFP-V5
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. LoxP1-Mb2YFP-2A (SO244)

    Plasmid

    #99613

    Purpose

    To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2EYFP
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. MLM3739

    Plasmid

    #49959

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CDmut HB-4 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

20. U6-hGRIN2B-CAG-ps-SaCas9

    Plasmid

    #102853

    Purpose

    A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. aureus)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Promoter

    U6 promoter;

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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