We narrowed to 62,288 results for: cloning

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pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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SP1-SB100X

Plasmid

#154887

Purpose

Sleeping Beauty transposase fused to SP1

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

SP1-Sleeping Beauty

Expression

Mammalian

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 CMV 3xFG eIF4G1

Plasmid

#158775

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:dCas9:nls:10P3

Plasmid

#135982

Purpose

Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

dCas9 fused to 10 copies of the P3 coiled-coil

Use

CRISPR

Tags

His

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Lyn11-FRB

Plasmid

#155228

Purpose

membrane anchor for FKBP-FRB system

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system

Expression

Mammalian

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0med

Plasmid

#153286

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Has Service

AAV2

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0mut

Plasmid

#153287

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMP71-hPDL-1

Plasmid

#118631

Purpose

human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

Depositor

Sébastien Walchli

Article

Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

Insert

hPDL-1 (CD274 Human)

Expression

Mammalian

Promoter

LTR

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KB

Plasmid

#139310

Purpose

Recruitable PI4KB

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

Plasmid

#139994

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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GRB2-V5

Plasmid

#139631

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

GRB2 (GRB2 Human)

Tags

3xV5-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Article

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-WT

Plasmid

#130700

Purpose

Expresses Beta-Globin WT NMD reporter

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCasTet-λ

Plasmid

#128318

Purpose

Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

Depositor

Christopher Johnston

Article

Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

Insert

TetR and pTetR/TetO

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Constitutive expression of cas9 and anhydro-tetra…

Promoter

tet promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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plx304-PHB-V5

Plasmid

#122230

Purpose

Expresses human PHB attached to a V5 tag

Depositor

Alex Kentsis

Article

Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

Insert

Prohibitin (PHB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti- V6.3 Ultra-Chili

Plasmid

#106173

Purpose

lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable

Depositor

Ewa Snaar-Jagalska

Insert

dTomato

Use

Lentiviral

Tags

T2A, P2A

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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Double UP mScarlet to mNeon

Plasmid

#125139

Purpose

Natively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pRC10-EFS-huLbCpf1-2A-Blast-WPRE

Plasmid

#123359

Purpose

Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS-NS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ArchT-BFP2-TSERex

Plasmid

#123312

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

ArchT-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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