We narrowed to 17,618 results for: eng
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Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
ExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-tetoff-mVenus-PICdc42
Plasmid#91876PurposePI(WT): Photo inhibitable (blue light sensitive)DepositorAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPS-7942
Plasmid#80459PurposeIPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942DepositorInsertSucrose phosphate synthase
ExpressionBacterialAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)
Plasmid#114086PurposeCAG promoter expression plasmid for human codon optimized LbBE1.4DepositorInserthuman codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationD832APromoterCAGAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-AncMEcoGII291
Plasmid#205024PurposeExpressing engineered M.EcoGII with higher thermostability and DNA methylation activityDepositorInsertAncestral M.EcoGII 291
TagsHis6x tagExpressionBacterialPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-ABE8e
Plasmid#206882PurposeExpresses FLAG-tagged ABE8e fused to Gag through a linker sequenceDepositorInsertABE8e
UseCRISPR and LentiviralTagsFLAGPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.12_GagZip-MCP-P2A-eGFP
Plasmid#205527PurposeExpress HIV GagZip-MCP-P2A-eGFPDepositorInsertGagZip-MCP
ExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.18_PB-SDC4-PGK-Blast
Plasmid#205563PurposeExpress human SDC4DepositorAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK1008 (pSL7)
Plasmid#191003PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_NES_HaloTag9
Plasmid#175537PurposeStrict cytosolic expression of HaloTag9 in mammalian cellsDepositorInsertNES-HaloTag9
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2369 - intron GG1 - 250bp
Plasmid#159877PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG1 - 250bp
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4
Plasmid#98975PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 4 and Cas9(D10A)
ExpressionMammalianAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
EKARdual
Plasmid#99870PurposeMeasure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertEKAR
TagsShadowG and mTFP1ExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PA(WT)-Vav2
Plasmid#86977PurposePI(DM): Photo-activatable/light sensitiveDepositorAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
VV201: ChR2(C128S)-eGFP in fubi
Plasmid#58510PurposeExpresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoterDepositorInsertChR2(C128S)-eGFP
UseLentiviralTagseGFPExpressionMammalianMutationChanged Cysteine 128 to SerinePromoterUbiquitinAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-CtermQ7
Plasmid#51442PurposeExpresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Q7 C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PTEN-H123Y
Plasmid#28299DepositorInsertPTEN (PTEN Human)
TagsFLAGExpressionMammalianMutationPhosphatase Inactive Mutant His123TyrAvailable sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLPC FLAG hRap1 delta CT
Plasmid#13252DepositorInsertRepressor Activator Protein 1 delta CT (TERF2IP Human)
UseRetroviralTagsFLAGMutationcontains hRap1 aa2-270 + 331-399 and the point mu…Available sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLPC FLAG hRap1 delta MC
Plasmid#13254DepositorInsertRepressor Activator Protein 1 delta MC (TERF2IP Human)
UseRetroviralTagsFLAGMutationcontains hRap1 aa2-131 + 265-399 and the point mu…Available sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
814 pSG5L HA PTEN 1-336
Plasmid#10742DepositorAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only