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We narrowed to 9,971 results for: 11

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Showing: 5261 - 5280 of 9971 results

  5. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Publication
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  6. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid
    #183876
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ANLN sgRNA spacer (ANLN Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    183876-plasmid-map-sequence-id-390709

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  7. pEPSW1KN0034

    Plasmid
    #196178
    Purpose
    MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT
    Depositor
    Nicola Patron
    Publication
    Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaMV35S::TMV::LucF::flag::35sT
    Use
    Synthetic Biology
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    196178-plasmid-map-sequence-id-386867

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  8. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid
    #183884
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB sgRNA spacer (ACTB Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183884-plasmid-map-sequence-id-362436

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  9. pODN-mR2-MYH10

    Plasmid
    #183869
    Purpose
    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183869-plasmid-map-sequence-id-362448

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  10. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid
    #183888
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TUBA1B sgRNA spacer (TUBA1B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183888-plasmid-map-sequence-id-362425

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  11. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid
    #183877
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RHOA sgRNA spacer (RHOA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183877-plasmid-map-sequence-id-362410

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  13. Flag-M4-AR-W741C-S81A

    Plasmid
    #171248
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171248-plasmid-map-sequence-id-336016

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  14. Flag-M4-AR-W741C-S81D

    Plasmid
    #171249
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171249-plasmid-map-sequence-id-336007

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  15. pcDNA3-AMPKsub-YPet-Kras

    Plasmid
    #84638
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AMPKsub-YPet-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, T…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    84638-plasmid-map-sequence-id-176882

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  16. LifeAct-TagBFP-DiB-RM

    Plasmid
    #168782
    Purpose
    Actin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RM
    Depositor
    Jens Meiler
    Publication
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LifeAct-TagBFP-DiB-RM
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168782-plasmid-map-sequence-id-332741

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Showing: 5261 - 5280 of 9971 results