We narrowed to 166,761 results for: Gene

1. LoxP3-HA-H2B-Cerulean-2A (SO104)

   Plasmid

   #99618

   Purpose

   To clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2A

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   HA-H2B-Cerulean
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MLM3720

   Plasmid

   #49944

   Purpose

   Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x FLAG Tet1CDmut RH-3 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Mutation

   catalytic domain of TET1 inactive (H1671Y and D16…

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. lentiCRISPR-v2-Blast-Puro

   Plasmid

   #167186

   Purpose

   Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LoxP3-Mb2-HA-mTFP1-2A (SO157)

   Plasmid

   #99615

   Purpose

   To clone gene of interest downstream of LoxP3-Mb2-HA-mTFP1-2A

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2-HA-mTfp1
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MLM3709

   Plasmid

   #49943

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x FLAG Tet1CD RH-3 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

6. iMb-Control-Mosaic (IR98.10)

   Plasmid

   #99748

   Purpose

   Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   MbYFP, MbTomato, MbKate2
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MLM3747

   Plasmid

   #49965

   Purpose

   Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x Flag Tet1CDmut HB-6 (HBB Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Mutation

   catalytic domain of TET1 inactive (H1671Y and D16…

   Promoter

   EF1a

   Available Since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

8. MLM3743

   Plasmid

   #49962

   Purpose

   Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x Flag Tet1CDmut HB-5 (HBB Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Mutation

   catalytic domain of TET1 inactive (H1671Y and D16…

   Promoter

   EF1a

   Available Since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

9. MLM3713

   Plasmid

   #49946

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x FLAG Tet1CD RH-4 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Promoter

   EF1a

   Available Since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

10. KI(GAPDH)-P2A-EGFP-CMV-NeoR

    Plasmid

    #114009

    Purpose

    HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection

    Depositor

    Pavel Volchkov

    Article

    Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126

    Inserts

    GAPDH left arm (GAPDH Chicken)
    eGFP
    NeoR
    GAPDH right arm
    

    Expression

    Mammalian

    Promoter

    -, CMV, and endogenous promoter (chicken GAPDH in…

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MLM3762

    Plasmid

    #49947

    Purpose

    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x FLAG Tet1CDmut RH-4 (RHOXF2 Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Mutation

    catalytic domain of TET1 inactive (H1671Y and D16…

    Promoter

    EF1a

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid

    #140193

    Purpose

    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    

    Use

    CRISPR and Synthetic Biology; Fungal genome integ…

    Tags

    VPR (VP64-p65-Rta), NLS

    Mutation

    D832A DNAse deactivated

    Promoter

    PgpdA and PtrpC

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgNotch2#2/Cre

    Plasmid

    #193226

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch2#2 (Notch2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. NC4 pCDNA3.1 VENUS WTX (1-804)

    Plasmid

    #36959

    Depositor

    Randall Moon

    Article

    Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

    Insert

    WTX (AMER1 Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

15. JA412

    Plasmid

    #49935

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD KL-1 (KLF4 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pSpCas9-Puro HLAG-2B-sgRNA

    Plasmid

    #182552

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2B-sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SIN-CMV-Cas9-V5-WPRE

    Plasmid

    #87904

    Purpose

    To produce lentiviral vector for gene editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    Cas9-V5
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MLM3727

    Plasmid

    #49961

    Purpose

    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    3x Flag Tet1CD HB-5 (HBB Human)
    

    Use

    TALEN

    Tags

    3x FLAG

    Promoter

    EF1a

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

    Plasmid

    #163138

    Purpose

    Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p426_Cas9_gRNA-R-ARS607c

    Plasmid

    #87409

    Purpose

    p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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