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We narrowed to 63,044 results for: cloning

Showing: 5261 - 5280 of 63044 results
  1. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

    Plasmid
    #211942
    Purpose
    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Expression
    Mammalian
    Promoter
    HSP68
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  2. ER-B

    Plasmid
    #209870
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Insert
    ddGFP B
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. iF59 PB-TO-oNgn2-puro

    Plasmid
    #204734
    Purpose
    Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.
    Insert
    Optimized neurogenin-2
    Expression
    Mammalian
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pAK_DR30_EF1a_CasRx_Puro

    Plasmid
    #134842
    Purpose
    Lentiviral all in one expression of CasRx and guideRNA
    Depositor
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 5261 - 5280 of 63044 results