We narrowed to 15,356 results for: nts

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCMB-TMEg

Plasmid

#61209

Purpose

Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-GFP-BCP

Tags

EGFP

Expression

Plant

Promoter

MtBCP1

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYes2-AtPCS1

Plasmid

#49766

Purpose

Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Article

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

AtPCS1 (PCS1 Mustard Weed)

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pAT9749-dABE

Plasmid

#162998

Purpose

plasmid expressing ABE with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dABE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDG279

Plasmid

#85219

Purpose

mammalian expression of ubiquitin-K48R-EGFP fusion

Depositor

Douglas Gray

Article

Tsirigotis et al J Biol Chem. 2001 Dec 7;276(49):46073-8.

Insert

ubiquitin C (UBC Human)

Tags

6xHIS and EGFP

Expression

Mammalian

Mutation

K48R

Promoter

human ubiquitin C

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
PI(LM)-Rac1(WT)dc1.FLARE

Plasmid

#87590

Purpose

PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET:CCCT:GFP

Plasmid

#60738

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAT15002-ABEmax

Plasmid

#163006

Purpose

plasmid expressing ABEmax

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

ABEmax

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15064-eCBE

Plasmid

#163009

Purpose

plasmid expressing eCBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

eCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0040

Plasmid

#117607

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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CBM9-N

Plasmid

#171710

Purpose

Expresses SARS-CoV-2 Nucleocapsid protein epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Nucleocapsid (N )

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-(PT4)P

Plasmid

#171708

Purpose

Carries CBM9 fusion tag

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

Cellulose Bindind Protein 9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRU205

Plasmid

#167685

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMIG small T C97S E102Q

Plasmid

#9060

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 97, 102 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

C97S, E102Q

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pEE386

Plasmid

#149284

Purpose

T-DNA encoding TRV2 with gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEE388

Plasmid

#149286

Purpose

T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_mFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only