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TagBFP2-C1-FKB-PI-PLCN187

Plasmid

#139307

Purpose

N-terminal half of split PI-PLC

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

TagBFP2:FKBP1A(3-108):[GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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iRFP-N1-PI-PLCC100-FRB

Plasmid

#139306

Purpose

C-terminal half of split PI-PLC

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

L. monocytogenes PI-PLC(188-287):IGTAGPRSANS[GA]4: MTOR(2021-2113):iRFP

Expression

Mammalian

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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Cx55.5:rasGFP

Plasmid

#105939

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Tags

GFP

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP Glra2 KA (CC#303)

Plasmid

#15201

Depositor

Connie Cepko

Article

Young et al Neuron. 2004 Mar 25. 41(6):867-79.

Insert

Glra2 (GLRA2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K310A

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA HA FAK Y576/577F

Plasmid

#50505

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y576/577F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
Kohinoor-Tublin/pcDNA3

Plasmid

#67780

Purpose

Tublin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

b-Tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
pNIC28-BpGmhA

Plasmid

#125589

Purpose

Overexpression plasmid for production of GmhA from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Harmer et al J Mol Biol. 2010 Jul 16;400(3):379-92. d

Insert

BpGmhA (gmhA Burkholderia pseudomallei)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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C-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45222

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

C-term Hec1-targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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mCherryC1-PIPKI{gamma}661

Plasmid

#29584

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Tags

mCherry

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pEF‐PIK3IP1

Plasmid

#49214

Purpose

expresses PIK3IP1 in mammalian cells

Depositor

Lawrence Kane

Article

DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

Insert

PIK3IP1 (Pik3ip1 Mouse)

Tags

6X-his and myc

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
Kohinoor-Clathrin

Plasmid

#67773

Purpose

Clathrin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Clathrin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 RNAiR2

Plasmid

#51585

Purpose

expresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSARSF505

Plasmid

#46832

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Tags

GFP_C and H6-GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

T7

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSopBWT

Plasmid

#112512

Purpose

PsopB driven sopB and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB/sigE operon

Expression

Bacterial

Promoter

PsopB

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-6-FLuc-9

Plasmid

#197737

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Firefly luciferase

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTagRFP675-actin-C1

Plasmid

#44277

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

Actin

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Kohinoor-mitochondria/pcDNA3

Plasmid

#67778

Purpose

Mitochondria-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

CoxVIII x 2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-FRB-LplA(W37I)

Plasmid

#34840

Depositor

Alice Ting

Article

Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

Insert

FRB-LplA(W37I)

Tags

FLAG (internal fusion), HA, and His Tag

Expression

Mammalian

Mutation

LplA has Trp37 to Val mutation

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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Kohinoor-H2B/pcDNA3

Plasmid

#67774

Purpose

Histon2B-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

H2B

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
EB3-eGFP-2xC2-iLID

Plasmid

#114419

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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