We narrowed to 14,179 results for: CRISPR-Cas9

Showing: 5301 - 5320 of 14179 results

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pENTR-PpU6P-sgRNA-L5L4

Plasmid

#113741

Purpose

Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1R5

Plasmid

#113737

Purpose

Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199485

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB8792

Plasmid

#126910

Purpose

Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ147

Plasmid

#69299

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro.3xBsmBI

Plasmid

#196709

Purpose

Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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EC70128

Plasmid

#209489

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC70214

Plasmid

#209490

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70125

Plasmid

#209486

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70127

Plasmid

#209488

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70126

Plasmid

#209487

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70212

Plasmid

#209484

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70213

Plasmid

#209485

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199484

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAEF6

Plasmid

#136303

Purpose

pAEF5 plasmid with two gRNA inserted

Depositor

Gilles Fischer

Article

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Insert

double cassette coding for gRNA

Use

CRISPR; Template for pcr to insert double grna co…

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

Plasmid

#99696

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

Depositor

George Church

Insert

dCas9 and gRNA targeting Afp (Afp Synthetic)

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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EC67900

Plasmid

#209494

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC70210

Plasmid

#209478

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70123

Plasmid

#209481

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge