We narrowed to 15,824 results for: LEA

1. pCAGGS-Twin-strep-TEV-DGKδ2

   Plasmid

   #223720

   Purpose

   Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted start codon

   Promoter

   CAG and chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-PHOSPHO1-TEV-Twin-Strep

   Plasmid

   #222015

   Purpose

   Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   stop codon (TGA) is deleted

   Promoter

   chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRRv2_sgTom

   Plasmid

   #218346

   Purpose

   CRISPR KO plasmid for tdTomato in human cell lines

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   Inserted sgRNA targetting tdTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac SOX 318-320A

   Plasmid

   #192060

   Purpose

   Expression vector for SOX (ORF37) 318-320A

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (ORF37)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   318-320A

   Promoter

   Polyhedrin

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDC Renilla Luc SRE

   Plasmid

   #191657

   Purpose

   Expressed Renilla Luciferase with SOX resistance element

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   Renilla Luciferase SRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SOX D221A/E244A/K246A pACEmam1

   Plasmid

   #192050

   Purpose

   Expresses a 3X catalytic mutant of SOX (ORF37)

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (3X catalytic mutant)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   3X catalytic mutant D221A/E244A/K246A

   Promoter

   CMV

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACE SOX (ORF37) K246A

   Plasmid

   #192051

   Purpose

   Expresses a catalytic mutant of SOX (K246A)

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (K246A)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Catalytic mutant K246A

   Promoter

   CMV

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACE SOX (ORF37) K250A

   Plasmid

   #192052

   Purpose

   Expresses a DNA binding mutant K250A (ORF37)

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (K250A)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   DNA binding mutant K250A

   Promoter

   CMV

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACE SOX (ORF37) 318-320A

   Plasmid

   #192054

   Purpose

   Expresses a mutant of SOX 318-320A

   Depositor

   Britt Glaunsinger

   Article

   Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

   Insert

   SOX (318-320A)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   SOX 318-320A

   Promoter

   CMV

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid

    #228724

    Purpose

    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

    Plasmid

    #228732

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid

    #228735

    Purpose

    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

    Plasmid

    #228731

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS2713-CMV-iNme2-ABE8e-i1-L10

    Plasmid

    #228734

    Purpose

    Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid iNme2 nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Mito(OM)-deltaCKAR

    Plasmid

    #222431

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Mitochondrial outer membrane delta-selective C kinase activity reporter
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-pDisplay-SNAP[C145A]

    Plasmid

    #223496

    Purpose

    Expression of dead-mutant SNAP-tags to outer surface in mammalian cells

    Depositor

    James Frank

    Article

    Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

    Insert

    dead-mutant SNAP-tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SNAP-tag C145A

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-HGM (optimized)

    Plasmid

    #220988

    Purpose

    Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

    Depositor

    Joshua Kritzer

    Article

    Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

    Insert

    HaloTag-GFP-mito
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT/T0-eGFP-NES-NFATC2IP

    Plasmid

    #221446

    Purpose

    Mammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Tags

    Nuclear Export Signal (NES) and eGFP

    Expression

    Mammalian

    Mutation

    NES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA5-FRT/T0-3XFLAG-NES-NFATC2IP

    Plasmid

    #221444

    Purpose

    Mammalian expression vector of triple Flag tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Tags

    3XFlag and Nuclear Export Signal (NES)

    Expression

    Mammalian

    Mutation

    NES (NLVDLQKKLEELELDEQQ)inserted, 1st Methionine …

    Promoter

    CMV

    Available since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pEN-X-mPGK-NeoR-SV40 polyA-Z

    Plasmid

    #218909

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    mPGK-Neomycine-SV40 polyA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    395T>C

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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