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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 47,629 results for: CHI;

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Showing: 5341 - 5360 of 47629 results

  3. pelB-MBP-DipA-ss

    Plasmid
    #137068
    Purpose
    E. coli expression clone (T7lac promoter) for mature DipA (aa 21-342) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature DipA: lacks the DipA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137068-plasmid-map-sequence-id-291958

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  4. pelB-MBP-RevA-ss

    Plasmid
    #137064
    Purpose
    E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    lacks the RevA signal peptide and 5 residues (CKA…
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137064-plasmid-map-sequence-id-291953

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  7. pGL3-Enhancer-ZC3H12D_PromoterMUT

    Plasmid
    #153068
    Purpose
    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter
    Depositor
    Silvia Monticelli
    Article
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Mutagenesis to disrupt 3 BHLHE40 binding sites. N…
    Promoter
    None
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    153068-plasmid-map-sequence-id-304889

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  8. pCDF-casBCDE(+6)

    Plasmid
    #89728
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.
    Depositor
    Konstantin Kuznedelov, Konstantin Severinov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a longer versi…
    Available Since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89728-plasmid-map-sequence-id-177602

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  10. pPBbsr2-EREG-ScNeo

    Plasmid
    #209909
    Purpose
    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EREG-ScNeo (EREG Human)
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209909-plasmid-map-sequence-id-416692

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  14. pPBbleo-HBEGF-ScNeo

    Plasmid
    #209903
    Purpose
    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    HBEGF-ScNeo (HBEGF Human)
    Tags
    mNeonGreen and mScarlet
    Expression
    Mammalian
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209903-plasmid-map-sequence-id-416658

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  15. pPBbsr2-HBEGF-ScNeo

    Plasmid
    #209907
    Purpose
    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    HBEGF-ScNeo (HBEGF Human)
    Tags
    mNeonGreen and mScarlet
    Expression
    Mammalian
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209907-plasmid-map-sequence-id-416690

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Showing: 5341 - 5360 of 47629 results