We narrowed to 20,057 results for: omp

1. pMR-A-3-Sasg

   Plasmid

   #214349

   Purpose

   Expresses T7-RNAP, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   T7-RNAP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA19_sgRNA-C1_M13ps

   Plasmid

   #231411

   Purpose

   M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEVA26_sgRNA-A0_M13ps

   Plasmid

   #231413

   Purpose

   M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Insert

   sgRNA-A0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEVA47_dCas9-GFP_NOT_iA

   Plasmid

   #231417

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEVA19_sgRNA-C0_M13ps

   Plasmid

   #231410

   Purpose

   M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-C0
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pU6-BoxB-petracrRNA

   Plasmid

   #207625

   Purpose

   Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   BoxB-petracrRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-giantin-ATG_donor

   Plasmid

   #222316

   Purpose

   Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

   Insert

   EGFP and homology arms for giantin/GOLGB1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSEVA47_dCas9-GFP_YES_i2

   Plasmid

   #231419

   Purpose

   This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   sgRNA-Y
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEVA47_dCas9-GFP_NOT_i1

   Plasmid

   #231416

   Purpose

   This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

   Depositor

   Manish Kushwaha

   Article

   Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

   Inserts

   dCas9
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTYB1-T4gp47

    Plasmid

    #228212

    Purpose

    Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

    Depositor

    Stephen Benkovic

    Article

    Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

    Insert

    47 (47 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-BirA-HA3-HIS3MX6

    Plasmid

    #227975

    Purpose

    To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BirA
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS416-CUP1p-TID-FRB-HA3

    Plasmid

    #228008

    Purpose

    Expresses TurboID-FRB fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS416-CUP1p-TID-FRB-NLS-HA3

    Plasmid

    #228009

    Purpose

    Expresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS416-CUP1p-TID-FKBP-HA3

    Plasmid

    #228010

    Purpose

    Expresses TurboID-FKBP fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS416-CUP1p- TID-FKBP-NLS-HA3

    Plasmid

    #228011

    Purpose

    Expresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS416-CHA1p-TID-FRB-HA3

    Plasmid

    #228012

    Purpose

    Expresses TurboID-FRB fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS416-CHA1p-TID-FRB-NLS-HA3

    Plasmid

    #228013

    Purpose

    Expresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FRB
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS416-CHA1p- TID-FKBP-NLS-HA3

    Plasmid

    #228015

    Purpose

    Expresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    CHA1

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-BioID2-HA3-HIS3MX6

    Plasmid

    #227977

    Purpose

    To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BioID3
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFa6-BASU-HA3-HIS3MX

    Plasmid

    #227979

    Purpose

    To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    BASU
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge