We narrowed to 6,058 results for: cat.2

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pSCL.176

Plasmid

#184997

Purpose

Express -Eco1 RNF2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Tags

SV40NLS

Expression

Mammalian

Mutation

RNF2 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.110

Plasmid

#184979

Purpose

Express -Eco1 TRP2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12

Expression

Yeast

Mutation

TRP2 donor E64stop

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sox3gRNA-T2

Plasmid

#127778

Purpose

Chicken sox3 gRNA target site 2, cloned into the pU6 sgRNA (backbone #92359)

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox3gRNAT2

Use

CRISPR

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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mOrange-imp α

Plasmid

#119720

Purpose

Expresses mOrange-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importinα (KPNA2 Human)

Tags

mOrange

Expression

Mammalian

Mutation

contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2m

Plasmid

#105066

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2s

Plasmid

#105067

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2s

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2.1s

Plasmid

#105068

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.1s

Tags

6x His tag

Expression

Bacterial

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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pJL1-FETCH1-StrepII

Plasmid

#210648

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH1

Use

Synthetic Biology

Tags

Strep-TagII

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR-SUMO1

Plasmid

#114388

Purpose

pDONR vector with SUMO1 gene

Depositor

Vincent Dion

Article

Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

Insert

SUMO1 (SUMO1 Human)

Expression

Mammalian

Mutation

Q29H compared with NCBI reference NP_003343.1

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-18 TC-sfGFP

Plasmid

#194632

Purpose

fluorescent protein with tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-sfGFP

Tags

MGCCPGCCGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Pgr_piwi-1

Plasmid

#107653

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

piwi-1

Use

RNAi; In situ hybridization

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pH6-mNeon

Plasmid

#178460

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTatABCmNeon

Plasmid

#178465

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatCmNeon

Tags

mNeonGreen fused to TatC

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLPP6

Plasmid

#209968

Purpose

Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter Dropout (sfGFP)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-Rabep1 shRNA 369958-CMV-FL Rabep1

Plasmid

#254779

Purpose

Rabep1 shRNA 369958 with expression of full length Rabep1 rescue in pLKO backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

shRNA 369958 and Rabep1 (RABEP1 Human)

Use

Lentiviral

Promoter

CMV

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Arhgap20-guides

Plasmid

#254763

Purpose

Arhgap20 gRNA for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Article

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Arhgap20 (arhgap20 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-HMP1_FABD

Plasmid

#186436

Purpose

Expresses the HMP1 F-actin Binding domain (677-927) with an N-terminal GST tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

HMP1 (F-actin Binding Domain) (hmp-1 Nematode)

Tags

GST

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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D38_Ld_341960

Plasmid

#135852

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

thioredoxin, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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D78_Ld_362280

Plasmid

#135891

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

transmembrane emp24 domain-containing protein

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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