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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,143 results for: Xpa

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Showing: 521 - 540 of 4143 results

  2. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Publication
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107999-plasmid-map-sequence-id-217151

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  3. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Publication
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107999-plasmid-map-sequence-id-217151

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  5. pS548∙CsR

    Plasmid
    #197859
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Tc resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197859-plasmid-map-sequence-id-393256

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  6. pS648∙CsR

    Plasmid
    #197860
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Gm resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197860-plasmid-map-sequence-id-402537

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  7. pS548∙CsR

    Plasmid
    #197859
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Tc resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197859-plasmid-map-sequence-id-393256

    Enlarge

  8. pS648∙CsR

    Plasmid
    #197860
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Gm resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197860-plasmid-map-sequence-id-402537

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  11. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enRhCas12f1
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197027-plasmid-map-sequence-id-388964

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  12. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  13. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enRhCas12f1
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197027-plasmid-map-sequence-id-388964

    Enlarge

  14. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  18. pCfB2399

    Plasmid
    #67550
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Publication
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    67550-plasmid-map-sequence-id-116387

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Showing: 521 - 540 of 4143 results