Addgene

We narrowed to 6,093 results for: cat.2

Showing: 521 - 540 of 6093 results

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TMEM230-WT-DsRed(isoform 2)

Plasmid

#99563

Purpose

mammalian expression of TMEM230-dsRed (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

DsRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 2)

Plasmid

#99562

Purpose

mammalian expression of TMEM230-GFP (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-KDM4A double Tudor

Plasmid

#59699

Purpose

Contains histone modification interacting domain (KDM4A double Tudor)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

KDM4A double Tudor (KDM4A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein beta-2

Plasmid

#67017

Purpose

G protein beta-2 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

NM_005273.3 (GNB2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein gamma-2

Plasmid

#67018

Purpose

G protein gamma-2 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-2 (GNG2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-MKL1/2 shRNA

Plasmid

#27161

Purpose

Lentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2.

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

shRNA MKL1+2

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-FOXM1-shRNA-#2

Plasmid

#89499

Purpose

Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

Depositor

Adam Karpf

Insert

FOXM1 (FOXM1 Human)

Use

Lentiviral and RNAi; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Spike-d18

Plasmid

#164583

Purpose

A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

Depositor

Jason Sheltzer

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Use

Tags

Expression

Mammalian

Mutation

Deleted amino acids 1256-1273

Promoter

EF1a

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pGEX6-2 SNAP-Syk tSH2

Plasmid

#113028

Purpose

for purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

SNAP-Syk tSH2

Use

Tags

SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP nLAP -2

Plasmid

#194507

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP +2

Plasmid

#194510

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP nLAP +2

Plasmid

#194316

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP -2

Plasmid

#194513

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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PX459v2-ILK-gRNA 2-exon 8

Plasmid

#163321

Purpose

SpCas9 ILK gRNA 2 (G*ACATTGTAGAGGGATCCATA), targeting exon 8 within the C-terminal protein kinase domain.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ILK gRNA 2_Exon8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6F

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pLKO.1 puro shPRDM14-2

Plasmid

#193695

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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p8224 pLIX-PTPN14 ΔPPXY1/2

Plasmid

#221644

Purpose

Doxycycline-inducible overexpression of human PTPN14 ΔPPXY1/2 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY1/2 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

deletion of the first and second (tandem) PPXY mo…

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_TJP1-1/1-SV2/2

Plasmid

#91494

Purpose

Protein expression and purification of human SH3 domain construct TJP1-1/1-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP1-1/1-SV2/2 (TJP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_TJP1-1/1-SV1/2

Plasmid

#91321

Purpose

Protein expression and purification of human SH3 domain construct TJP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP1-1/1-SV1/2 (TJP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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