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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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Triple ORF FRT ifgMosaic Donor (IG135)

Plasmid

#99626

Purpose

To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

PGK-Neo

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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4xnr UAS ifgMosaic Donor vector (IG230)

Plasmid

#99631

Purpose

To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Triple ORF FRT ifgMosaic Donor (IG135)

Plasmid

#99626

Purpose

To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

PGK-Neo

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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4xnr UAS ifgMosaic Donor vector (IG230)

Plasmid

#99631

Purpose

To clone the 3 ORFs for Gal4 dependent generation of ifgMosaics

Depositor

Rui Benedito

Publication

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

N-PhiM

Use

Cre/Lox

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Publication

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Publication

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TvMT Thermosynechococcus vulcanus Metallothionein

Plasmid

#200299

Purpose

Bacterial expression plasmids for production of recombinant Thermosynechococcus vulcanus Metallothionein

Depositor

Artur Krężel

Publication

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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TvMT Thermosynechococcus vulcanus Metallothionein

Plasmid

#200299

Purpose

Bacterial expression plasmids for production of recombinant Thermosynechococcus vulcanus Metallothionein

Depositor

Artur Krężel

Publication

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmOskar-PC_Q

Plasmid

#147323

Purpose

Bacterial Expression of DmOskar-PC

Depositor

Elisa Izaurralde

Insert

DmOskar-PC (osk Fly)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmOskar-PC_Q

Plasmid

#147323

Purpose

Bacterial Expression of DmOskar-PC

Depositor

Elisa Izaurralde

Insert

DmOskar-PC (osk Fly)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Publication

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Expression

Worm

Promoter

myo-2

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Publication

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Expression

Worm

Promoter

myo-2

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 RD/LD [282-490]

Plasmid

#186470

Purpose

Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [282-490]

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 RD/LD [282-490]

Plasmid

#186470

Purpose

Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [282-490]

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBACgus_Sumostar/LRRK2 1867-2141

Plasmid

#40390

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2141

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus_Sumostar/LRRK2 1867-2161

Plasmid

#40391

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2161

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus_Sumostar/LRRK2 1867-2161_G2019S

Plasmid

#40393

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2161, G2019S

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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