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Plasmid#78481Purpose600nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
miniUAA3
Plasmid#78482Purpose882nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG/MNase
Plasmid#123461PurposeExpresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.DepositorInsertProtein A-Protein G-Micrococcal Nuclease
UseTags6xHIS and HAExpressionBacterialMutationPromoterLacAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM-intron 3x UAS attB
Plasmid#206360Purposeincludes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequencesDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(1)_T2ALexA
Plasmid#125221PurposeArtificial phase 1 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertLexA
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(0)_T2ALexA
Plasmid#125220PurposeArtificial phase 0 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertLexA
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2ALexA
Plasmid#125222PurposeArtificial phase 2 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertLexA
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p102_gRNA_Sa_mClover
Plasmid#213778PurposeCloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.DepositorInsertSa gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
p103_gRNA_Sp_mClover
Plasmid#213779PurposeCloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.DepositorInsertSp gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-APEX2-P2A-EGFP
Plasmid#108896PurposeMultisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertAPEX2-P2A-EGFP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRNA2-(A)128
Plasmid#174006PurposeUsed to generate stable mRNA for heterologous expression experiments (includes polyA tail)DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-puro
Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh
Plasmid#208276PurposeExpresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh
Plasmid#208277PurposeExpresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-TurboID-3MYC-kanMX6 (pFB1420)
Plasmid#126049PurposeFor C-terminal tagging with TurboID in yeast including geneticin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TurboID-3MYC-natMX6 (pFB1434)
Plasmid#126050PurposeFor C-terminal tagging with TurboID in yeast including nourseothricin-resistant markerDepositorInsertTurboID
UsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2ST
Plasmid#59384PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.DepositorInsertTT-ISceI-dfrA
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-hPPT1
Plasmid#85815PurposeExpression of hPPT in mammalian cellsDepositorInserthuman PPT1 (PPT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
12.4kbVillin-ΔATG
Plasmid#19358DepositorInsert12.4kb Villin promoter
UseTagsExpressionBacterialMutationNo ATG - transgenes cloned downstream of the vill…PromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only