We narrowed to 1,670 results for: nif

1. pET22b-ecDHFR-G51PEKN

   Plasmid

   #118582

   Purpose

   Bacterial expression of E.coli DHFR G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G51 mutated to P51; Glu-Lys-Asn residues inserted…

   Promoter

   T7

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST39-His/Vps27(345-440) - Hse(275-375)

   Plasmid

   #17638

   Depositor

   James Hurley

   Article

   Prag et al Dev Cell. 2007 Jun . 12(6):973-86.

   Insert

   His/Vps27(345-440) – Hse1(275-375) (VPS27, HSE1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

3. NFAST(low)-Halo-ER

   Plasmid

   #214419

   Purpose

   ER localization of low-affinity NFAST fused to Halo-ER

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspA-N
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC57-min5UTR-hRluc-A60

   Plasmid

   #182638

   Purpose

   Transcription template plasmid for a minimal 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

   Depositor

   Stephen Floor

   Article

   Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

   Insert

   Renilla luciferase,
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUiLMO2W

   Plasmid

   #129480

   Purpose

   3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

   Depositor

   Robert Gross

   Article

   Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

   Insert

   iLMO2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C

   Available Since

   Feb. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-iLMO2

   Plasmid

   #129474

   Purpose

   Mammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

   Depositor

   Robert Gross

   Article

   Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

   Insert

   iLMO2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ

   Plasmid

   #192399

   Purpose

   Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-Rluc-FRT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ

   Plasmid

   #192398

   Purpose

   On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-Rluc-FRT
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

   Plasmid

   #192373

   Purpose

   To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::FRT-OCS-TB-FRT::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC

    Plasmid

    #192376

    Purpose

    To test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-uORF-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC

    Plasmid

    #192370

    Purpose

    To control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ

    Plasmid

    #192375

    Purpose

    To test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-35Ster-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ

    Plasmid

    #192374

    Purpose

    To test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCStrunc-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ

    Plasmid

    #192385

    Purpose

    To test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ

    Plasmid

    #192386

    Purpose

    To test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192404

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192401

    Purpose

    Off state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ

    Plasmid

    #192400

    Purpose

    On state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::B3RT-Rluc-B3RT
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ

    Plasmid

    #192402

    Purpose

    On state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    FRT-Act2-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ

    Plasmid

    #192405

    Purpose

    Off state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    B3RT-Act2-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge