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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 952 results for: trna

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Showing: 521 - 540 of 952 results

  5. pcDNA3.1-flag-IARS2-Δ1-48

    Plasmid
    #232341
    Purpose
    Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340
    Depositor
    Quanfu Ma
    Insert
    IARS2 (IARS2 Human)
    Expression
    Mammalian
    Mutation
    Residues 1-48 (indicated as Mito-signal in UniPro…
    Promoter
    CMV
    Available Since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    232341-plasmid-map-sequence-id-474530

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  7. pOP004

    Plasmid
    #185985
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminator
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Expression
    Bacterial
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    185985-plasmid-map-sequence-id-378299

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  8. pOP005

    Plasmid
    #186030
    Purpose
    Encodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly downstream of the promoter
    Depositor
    Georgi Belogurov
    Article
    Huang et al Methods Enzymol. 2022;675:207-233. doi:
    Insert
    Bacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
    Expression
    Bacterial
    Available Since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186030-plasmid-map-sequence-id-371540

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  11. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-466939

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  14. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid
    #239293
    Purpose
    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system
    Depositor
    Anita Hopper
    Article
    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.
    Inserts
    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    Expression
    Yeast
    Mutation
    Contains M2-SE-G72P mutations, described in Addge…
    Promoter
    TDH3 and tetracycline-responsive element (TRE) an…
    Available Since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239293-plasmid-map-sequence-id-476828

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  15. pCMV-MbPylRS(DiZPK)

    Plasmid
    #91706
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5
    Inserts
    pylS
    pylT
    Expression
    Mammalian
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91706-plasmid-map-sequence-id-183279

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  20. pMOD_B2303

    Plasmid
    #91068
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Promoter
    CmYLCV
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91068-plasmid-map-sequence-id-181874

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Showing: 521 - 540 of 952 results