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Pk_P38-Cd4-bio-His

Plasmid

#126846

Purpose

Expression of recombinant P. knowlesi protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

P38 (PKH_102490 Plasmodium knowlesi)

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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Pm_P12-Cd4-bio-His

Plasmid

#126822

Purpose

Expression of recombinant P. malariae protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

P12 (P12p Plasmodium malariae)

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

Plasmid

#182381

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-bPAC(R278A)-Myc-eYFP

Plasmid

#165488

Purpose

Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

bPAC(R278A)-Myc-eYFP

Use

Cdna expression, xenopus oocyte

Tags

EYFP and Myc

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDM304_YFP-IqgC

Plasmid

#128697

Purpose

expression of YFP-tagged IqgC in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

Insert

iqgC

Use

Dictyostelium discoideum expression vector

Tags

YFP

Mutation

none

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR359.4

Plasmid

#125115

Purpose

Expression of narL(1-159aa)-uhpA(142-129aa) chimera under PLtetO-1, output Promoter PuhpT99

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-uhpA(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pKD275

Plasmid

#125097

Purpose

Expression of SO_0622(1-129aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_0622(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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PCRII apoA-IVa

Plasmid

#121902

Purpose

Plasmid for making zebrafish apoA-IVa in situ probe

Depositor

Steven Farber

Article

Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

Insert

ApoA4a (apoa4a Zebrafish)

Use

Unspecified

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP

Plasmid

#120911

Purpose

Expresses BFP tagged Ptch1-B (V111FL114FI766F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA and TagBFP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP

Plasmid

#120908

Purpose

Expresses Ptch1-B (V111FL114FW115A)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP

Plasmid

#120907

Purpose

Expresses Ptch1-B (I766FV111F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-Y233C-HA

Plasmid

#120904

Purpose

Expresses Ptch1-B (Y233C) for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-L234C-HA

Plasmid

#120905

Purpose

Expresses Ptch1-B (L234C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-C mSesn2 (delAB)

Plasmid

#111799

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

AB domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-B+C mSesn2 (delA)

Plasmid

#111798

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-A mSesn2 (delBC)

Plasmid

#111797

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

BC domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-3/4

Plasmid

#91359

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-3/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-3/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-2/4

Plasmid

#91316

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-2/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-2/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-1/4

Plasmid

#91317

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-1/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-1/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2B-4/4

Plasmid

#91257

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2B-4/4

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2B-4/4 (SH3PXD2B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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