We narrowed to 13,411 results for: BASE
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Plasmid#141013PurposeMutisite Gateway mediated vector constructionDepositorInsertCas9
ExpressionMammalianAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM1
Plasmid#133632PurposeIntegration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM103
Plasmid#133664PurposeIntegration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
library backbone 3'LTR gRNA and barcode
Plasmid#127397PurposeDigestion by BstXI and BamHI to release the backboneDepositorTypeEmpty backboneUseLentiviralTagsPuro-mCherryAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HLA-cmyc-optiT1R3 a21-852
Plasmid#113948Purposemammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationresidues 21-852PromoterCMVAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
VKG1-gRNA-pX330
Plasmid#108671PurposeCleavage of VKG1 sequence by CRISPR/Cas9DepositorInsertgRNA for VKG1 sequence
UseCRISPRAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:S10-(MCS)-3xHA
Plasmid#108183PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterP16ΔSAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))
Plasmid#105210PurposeModule 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)DepositorInsertSSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)
UseSynthetic BiologyExpressionMammalianAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)
Plasmid#70210Purpose1 Part of 4-part switchDepositorInsertdeGFP
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)
Plasmid#49065PurposeExpresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutation2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …PromoterCMVAvailable SinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)
Plasmid#49063PurposeExpresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutation2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …PromoterCMVAvailable SinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLP110_AAV Scramble Control
Plasmid#239418PurposeNegative control AAV vector for SaCas9-based CRISPR KODepositorInsertScramble control
UseAAVAvailable SinceApril 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLP17_Lenti α-syn
Plasmid#239419PurposeLentiviral plasmid for SpCas9-based CRISPR KO of mouse α-synDepositorInsertmouse a-synuclein sgRNA (Snca Mouse)
UseLentiviralAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-miniCGBE1
Plasmid#198538PurposeExpresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-miniCGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-MPro-eGFP-2
Plasmid#184824PurposeExpresses Mpro-eGFP in mammalian cellsDepositorInsertMpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)
UseLentiviralExpressionMammalianPromoterEF-1alfaAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B5
Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only