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CALM1 gRNA (BRDN0001146464)

Plasmid

#75763

Purpose

3rd generation lentiviral gRNA plasmid targeting human CALM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CALM1 (CALM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HRas (G12V, E37G)-pcw107

Plasmid

#64639

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (transcript variant 3) (HRAS Human)

Use

Lentiviral

Mutation

G12V, E37G

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
HRas (G12V, E37G)-pcw107-V5

Plasmid

#64640

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (transcript variant 3) (HRAS Human)

Use

Lentiviral

Tags

V5

Mutation

G12V, E37G

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAO139 (Apr LEU2 mrcl-AQ)

Plasmid

#41924

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Mutation

MRC1AQ

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pAO122 (Apr LEU2 MRC1)

Plasmid

#41923

Depositor

Stephen Elledge

Article

Osborn et al Genes Dev. 2003 Jul 15;17(14):1755-67.

Insert

Mediator of the Replication Checkpoint

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-3(β2)

Plasmid

#160268

Purpose

Expresses a concatemeric construct of a α4:β2 (2:3) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

3(β2) concatemer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-3(α4)

Plasmid

#160267

Purpose

Expresses a concatemeric construct of a α4:β2 (3:2) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

3(α4) concatemer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSLB025

Plasmid

#234634

Purpose

pET-28a(+) based plasmid for expression of H16_A1358 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A1358

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-α5last

Plasmid

#160270

Purpose

Expresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

α5last concatemer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-α5first

Plasmid

#160269

Purpose

Expresses a concatemeric construct of a α4:β2:α5 (2:2:1) nAChR

Depositor

Pierre-Jean Corringer

Article

Prevost et al Cell Mol Life Sci. 2020 May 29. pii: 10.

Insert

α5first concatemer

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-I-SceI

Plasmid

#165041

Purpose

Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

I-SceI

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSLB027

Plasmid

#234636

Purpose

pET-28a(+) based plasmid for expression of H16_B1102 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1102 (H16_RS24270 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB028

Plasmid

#234637

Purpose

pET-28a(+) based plasmid for expression of H16_B1148 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1148 (H16_RS24495 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB029

Plasmid

#234638

Purpose

pET-28a(+) based plasmid for expression of H16_B1264 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1264 (H16_RS25065 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB030

Plasmid

#234639

Purpose

pET-28a(+) based plasmid for expression of H16_B1335 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1335 (H16_RS25400 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB026

Plasmid

#234635

Purpose

pET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A3514 (H16_RS17530 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF- RPA4 (MSW#716)

Plasmid

#208077

Purpose

Expression of human Replication Protein A 4 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4 (RPA4 Human)

Expression

Bacterial

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.MJ0130m

Plasmid

#11528

Depositor

Sung-Hou Kim

Article

Kim et al Proc Natl Acad Sci U S A. 2005 Mar 1. 10

Insert

Type I restriction-modification enzyme (MJ_0130m M. jannaschii)

Tags

HisTag, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Article

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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