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pAAV-UbC-eGFP

Plasmid

#62518

Purpose

Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

Depositor

Jae Lee, Pantelis Tsoulfas

Article

Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hUbC

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mRuby2-CCR5TC

Plasmid

#135531

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTagBFP2-blast

Plasmid

#125239

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTagBFP2 (codon optimized)

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-mNeonGreen-CCR5TC

Plasmid

#135533

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

mNeonGreen

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-EWS-FLI1

Plasmid

#237636

Purpose

For overexpression of mEGFP-EWS-FLI1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EWS-FLI1 (EWSR1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCF140_Gag-SpyCas9-NLS

Plasmid

#225959

Purpose

CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-Gag-SpyCas9-NLS

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-BRD4

Plasmid

#65378

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD4 (BRD4 Human)

Tags

EmGFP

Expression

Mammalian

Mutation

R1232W

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-ATF4 mScarlet NLS

Plasmid

#115969

Purpose

ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

activating transcription factor 4 (ATF4 Synthetic, Human)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-puro/Flag PD-L1 WT

Plasmid

#121446

Purpose

expression of huamn PD-L1 wildtype in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

wildtype

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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VPS13C^Halo

Plasmid

#232864

Purpose

Expresses human VPS13C^Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

VPS13C (VPS13C Human)

Tags

internal Halo

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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VPS13C^Halo-△(ATG2C-PH)

Plasmid

#232867

Purpose

Expresses VPS13C-△(ATG2C-PH) in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

VPS13C-△(ATG2C-PH) (VPS13C Human)

Tags

internal Halo

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-VAB(VPS13C)

Plasmid

#232865

Purpose

Expresses the VAB domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the VAB domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-SOD2-2A-Catalase-WPRE

Plasmid

#67635

Purpose

AAV vector expressing both SOD2 and mitochondrial targeted Catalase

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

SOD-2A-Catalase (Sod2, Cat Mouse)

Use

AAV

Mutation

Deleted the Peroxisome Targeting Signal from Cata…

Promoter

CMV

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN

Plasmid

#227433

Purpose

Expresses the R-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN (Pten Rat)

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-BRD3

Plasmid

#65377

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD3 (BRD3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-BAZ1B

Plasmid

#65372

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ1B (BAZ1B Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET iGluSnFR3 v857

Plasmid

#175186

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v857

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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