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ATAD2 D8.3 gRNA

Plasmid

#90539

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATAD2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ATAD2 (Guide Designation D8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pStr-KDEL_EPV-SBP-DsRed2-ER-5

Plasmid

#170676

Purpose

Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Inserts

EPV-SBP-DsRed2-ER-5
streptavidin-KDEL

Expression

Mammalian

Promoter

IRES and T7

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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WEE1 F10.4 gRNA

Plasmid

#90941

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F10.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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WEE1 F9.4 gRNA

Plasmid

#90940

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

WEE1 (Guide Designation F9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pQnl_tet

Plasmid

#191355

Purpose

Clostridium expression vector with TetR-repressed NanoLuc expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette

Expression

Bacterial

Promoter

PGus2A-TetO2/1

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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POT1 E3.4 gRNA

Plasmid

#90842

Purpose

3rd generation lentiviral gRNA plasmid targeting human POT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

POT1 (Guide Designation E3.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 A8.4 gRNA

Plasmid

#90897

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A8.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 A7.4 gRNA

Plasmid

#90896

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 H8.1 gRNA

Plasmid

#90895

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H8.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 H7.1 gRNA

Plasmid

#90894

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H7.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-1x

Plasmid

#124690

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

Plasmid

#52895

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pSLIK_Zeo_3xflag_Luciferase

Plasmid

#136533

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

Luciferase

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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CLTC G9.4 gRNA

Plasmid

#90634

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLTC

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CLTC (Guide Designation G9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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SLC19A1_pCSdest

Plasmid

#53948

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SLC19A1 (SLC19A1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Gata3-MIEG-hCD4

Plasmid

#40568

Depositor

Alexander Dent, Mark Kaplan

Article

Chang et al Immunity. 2005 Jun;22(6):693-703.

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

IRES hCD4

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
IGSF5_pCSdest

Plasmid

#53886

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IGSF5 (IGSF5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pCLXSN-HA-CYLD

Plasmid

#27556

Depositor

Shao-Cong Sun

Article

Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R156G and G179R

Available Since

Aug. 30, 2011

Availability

Academic Institutions and Nonprofits only
EGFP-Rab8a in pCSDEST2

Plasmid

#140880

Purpose

Expression of zebrafish GFP-tagged Rab8a in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab8a (rab8a Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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KIF11 F5.3 gRNA

Plasmid

#90716

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF11

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF11 (Guide Designation F5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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