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pFTP1

Plasmid

#64952

Purpose

source of TpR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-dhfrII-FRT

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDB4279

Plasmid

#98698

Purpose

A control plasmid containing the intact bsdMX marker

Depositor

Li-Lin Du

Article

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMS2-1

Plasmid

#220627

Purpose

Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBRIT TAP

Plasmid

#17520

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

TAP

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pMT-P4

Plasmid

#120866

Purpose

Encodes permuteposon P4 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P4

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMMK801

Plasmid

#207140

Purpose

Type 8 Tn10 backbone part, with RFP dropout

Depositor

Nicholas Shikuma

Article

Alker et al ISME Commun. 2023 Sep 19;3(1):98. doi: 1

Type

Empty backbone

Promoter

n/a

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-P2

Plasmid

#120864

Purpose

Encodes permuteposon P2 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P2

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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BB1_12_YlpGPD

Plasmid

#117820

Purpose

Promoter pGPD of Y. lipolytica for GoldenMOCS

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpGPD

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HisMS2_VLP_FluA

Plasmid

#175955

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Insert

M gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMT-P3

Plasmid

#120865

Purpose

Encodes permuteposon P3 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P3

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKM343

Plasmid

#71487

Purpose

Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-cat-loxP

Use

Source of loxp-hyg-cat- loxp for pcr amplificatio…

Expression

Bacterial

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDH22

Plasmid

#83798

Purpose

Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1_Puro_ccdb_Ubc_rtTA

Plasmid

#172130

Purpose

Destination vector for AAVS1 targeting

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Expression

Mammalian

Promoter

PGK1

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2313

Plasmid

#137053

Purpose

Endogenous Locus DiCre Expression Cassette

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Type

Empty backbone

Use

Donor vector for endogenous locus dicre

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBS4

Plasmid

#83793

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Tags

CFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pUBN-paGFP-Dest

Plasmid

#105108

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Tags

paGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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FRP1483

Plasmid

#64511

Purpose

Expresses destabilized Ametrine in yeast.

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

Ametrine-ADH1-tail

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMIG-RhoC

Plasmid

#14119

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

Human RhoC (RHOC Human)

Use

Retroviral

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pFS119

Plasmid

#12379

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

ade6, thymidine kinase

Expression

Yeast

Available Since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pBM5218

Plasmid

#18796

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Ty5-HIS3AI

Expression

Yeast

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only