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pWM_12x601_20bpLinker

Plasmid

#157787

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_20bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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Mut2-RS

Plasmid

#182882

Purpose

A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

Depositor

Miriam Amiram

Article

Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

Insert

Mut2-RS

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-EPSIN 1

Plasmid

#22228

Depositor

Pietro De Camilli

Article

Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

Insert

Epsin 1 (Epn1 Rat)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

please see depositor comment below

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pMpGE014

Plasmid

#108682

Purpose

All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

Depositor

Takayuki Kohchi, Shigeo Sugano

Article

Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

Type

Empty backbone

Use

CRISPR

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-3xFLAG-NLS-GFP

Plasmid

#198238

Purpose

Lentivector with tet-inducible 3xFLAG-NLS-GFP

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

3xFLAG-NLS-GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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AsCas12a-6xNLS-E174R/S542R (pRG232)

Plasmid

#149723

Purpose

Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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p415TEF cyto roGFP2-Grx1(yeast)

Plasmid

#65004

Purpose

Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1

Tags

roGFP2

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV4-p52

Plasmid

#23289

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

p52 (NFKB2 Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416L

Plasmid

#81036

Purpose

mammalian expression of LOV V416L, a mutant with super long relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416L

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416L

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL_U2AF1_WT_mCherry

Plasmid

#84017

Purpose

expression of U2AF1 in mammalian cells

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDAS12125_PEAR-GFP

Plasmid

#177178

Purpose

PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV LDTS-LIRmut

Plasmid

#189001

Purpose

lipophagy-inducing adaptor protein, negative control

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-LIRmut

Use

Retroviral

Tags

HA

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Jean-Baptiste Boulé, Virgina Zakian

Article

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Tags

histag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-Gal4-Vp16-Crys-CFP

Plasmid

#97144

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Gal4-Vp16

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_35bpLinker

Plasmid

#157790

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_35bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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H3K9me3Probe_FullLength

Plasmid

#186463

Purpose

Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

Depositor

Chongli Yuan

Article

Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

Insert

dimeric CDY1 (CDY1 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_15bpLinker

Plasmid

#157786

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 15 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_15bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-mmA1

Plasmid

#61090

Purpose

expression of fusion protein: MBP-hnRNP A1

Depositor

Sean Ryder

Article

Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

Insert

hnRNP A1 (Hnrnpa1 Mouse)

Tags

H6_MBP

Expression

Bacterial

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
SpyTag-β-Lactamase-SpyCatcher

Plasmid

#52656

Purpose

Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

Depositor

Mark Howarth

Article

Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

Insert

TEM-1 β-Lactamase

Tags

SpyCatcher and SpyTag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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