We narrowed to 6,021 results for: RIK

1. pCS2-HA-Mettl3

   Plasmid

   #196262

   Purpose

   In vitro transcription/translation of HA-tagged wild-type Mettl3

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   Mettl3 (Mettl3 Mouse)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Synaptotagmin-6 scFv [N270/47]

   Plasmid

   #190544

   Purpose

   Mammalian Expression of Synaptotagmin-6 scFV. Derived from hybridoma N270/47.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Synaptotagmin-6 (Mus musculus) recombinant scFV (Syt6 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

   Plasmid

   #105023

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfb (Cobra1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

   Plasmid

   #105024

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfb (Cobra1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS1830 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-U6-Fah

   Plasmid

   #199263

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAdCMV/V5-DEST-Y705F-STAT3-3xFlag

   Plasmid

   #99261

   Purpose

   Adenoviral vector encoding Flag-tagged murine STAT3 (Y705F)

   Depositor

   Alejandro Adam

   Article

   Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

   Insert

   Signal transducer and activator of transcription 3 - Y705F (Stat3 Mouse)
   

   Use

   Adenoviral

   Tags

   3x-Flag

   Mutation

   Y705F (contains a silent mutation: nucleotide 271…

   Promoter

   CMV

   Available Since

   Jan. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgCRY2

   Plasmid

   #197457

   Purpose

   Expresses sgRNA(CRY2 gene) in mammalian cells

   Depositor

   Han Kyoung Choe

   Article

   Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

   Insert

   hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)
   

   Use

   AAV

   Tags

   mCherry (K14E, N210D, E249G)

   Promoter

   U6, hSyn

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgSf3b1(T1)

   Plasmid

   #90424

   Purpose

   induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700

   Depositor

   Alex Minella

   Article

   Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

   Insert

   mus Sf3b1 gRNA (Sf3b1 Mouse)
   

   Use

   CRISPR; Guiderna encoding for gene editing

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR8/GW/TOPO-ELK3

   Plasmid

   #98622

   Purpose

   Gateway entry TOPO TA cloning vector for ELK3

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK3 (Elk3 Mouse)
   

   Use

   Topo ta cloning vector

   Mutation

   E167K

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLL5.0 ShCavin1-EGFP

    Plasmid

    #187253

    Purpose

    Lentiviral expression of shRNA targeting Cavin1

    Depositor

    Alpha Yap

    Article

    Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

    Insert

    ShRNA for Cavin1 (Cavin1 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCVpuro-F-Gcn5-NLSm

    Plasmid

    #63708

    Purpose

    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated K425, K427 and K438 to R (arginine)

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pPICZ:mTREK-1_cryst

    Plasmid

    #133269

    Purpose

    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-Hyg-Snrnp40-CRISPR-resistant

    Plasmid

    #134251

    Purpose

    Lentivector encoding CRISPR-resistant Snrnp40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    mutated coding sequence “gataactatgcgacgttgaa” to…

    Promoter

    EF1a

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Otx2 x2)

    Plasmid

    #171102

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Otx2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Otx2 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV2-TBG-FLAG-mRIPK1-S321A

    Plasmid

    #115342

    Purpose

    Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321A

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321A

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-myc-CXCR4-W161Y

    Plasmid

    #98951

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161Y

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    W161Y: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GP804

    Plasmid

    #164548

    Purpose

    Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    All sixteen Lysines in cytoplasmic C-terminal tai…

    Promoter

    GgCryD1

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEP4-myc-CXCR4-W161H

    Plasmid

    #98952

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CXCR4 mutant W161H

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    W161H: enhanced CXCL12 binding and signaling

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGal-Trans-Myc-HA-GFP11-KDEL

    Plasmid

    #177724

    Purpose

    ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
    

    Tags

    GFP11, HA, KDEL, and Myc

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBOB-N-3HA-GK_v1

    Plasmid

    #134275

    Purpose

    Lentivector encoding 3XHA-tagged GK (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    524 amino acids, isoform 1

    Promoter

    CMV

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge