We narrowed to 9,299 results for: yeast expression

1. LOV-Turbo-V5_pRS415

   Plasmid

   #199667

   Purpose

   expresses LOV-Turbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Tags

   V5

   Expression

   Yeast

   Mutation

   Promoter

   Gal1,10

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pME-puro-Venus-FLAG-CD59

   Plasmid

   #50377

   Purpose

   Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

   Depositor

   Reika Watanabe

   Article

   Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pDuRCC-N

   Plasmid

   #81194

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please view depositor comments below and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDFDuet-trAtCCD7-OsD27(pYL726 )

   Plasmid

   #178285

   Purpose

   Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Inserts

   OsD27
   trAtCCD7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTPI1-EMC1-GFP-APEX2-URA3

   Plasmid

   #184776

   Purpose

   Expresses EMC1-GFP-APEX2 chimera for labeling yeast endoplasmic reticulum (ER). Uses auxotrophic marker URA3(Kluyveromyces lactis).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   APEX2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJT43_GalL_BE-P(AP)3

   Plasmid

   #145036

   Purpose

   Expresses BE-P(AP)3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE-(AP)3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS405 PEX14-VAC8-RFP

   Plasmid

   #166844

   Purpose

   Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, PEX14 Budding Yeast)
   

   Use

   Tags

   PEX14 transmembrane domain and RFP

   Expression

   Yeast

   Mutation

   PEX14 transmembrane domain

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX4T2-Snf1-cat

   Plasmid

   #52683

   Purpose

   expression of the Snf1 kinase domain in bacteria

   Depositor

   Benjamin Turk

   Article

   Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

   Insert

   SNF1 (SNF1 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes kinase domain only, aa 41-315

   Promoter

   tac

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pRS406 ATG1-ATG17-3'UTR

    Plasmid

    #166848

    Purpose

    Expresses Atg1 with ATG17 3'UTR in yeasts.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ATG1 Promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS316-RGR-GFP-mm

    Plasmid

    #51059

    Purpose

    Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    RGR-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJKW1847

    Plasmid

    #174004

    Purpose

    Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

    Depositor

    Jing-Ke Weng

    Article

    Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

    Inserts

    PpCYP94D108
    ATR1 (ATR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJW3.42

    Plasmid

    #62866

    Purpose

    Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    PPTR-1/pLexA-N bait
    

    Use

    Two-hybrid bait vector

    Tags

    LexA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

    Plasmid

    #216404

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51, 56, Glutamine 70, 443 and Asp…

    Promoter

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC57-fsr1

    Plasmid

    #179866

    Purpose

    Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Insert

    Fusarubin polyketide synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTF1 deltaC12

    Plasmid

    #205068

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 12 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTF1 deltaC16

    Plasmid

    #205069

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 16 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTF1 deltaC18

    Plasmid

    #205070

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 18 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTF1 deltaC20

    Plasmid

    #205071

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 20 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTF1 deltaC9

    Plasmid

    #205067

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 9 amino acids

    Promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge