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pDL52 (clone76)

Plasmid

#188579

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

Plasmid

#226104

Purpose

Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

COX8A (COX8A Human)

Tags

GFP

Expression

Mammalian

Mutation

encodes only for mitochondrial targeting sequence…

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-PARP1

Plasmid

#215136

Purpose

Expresses mCherry-PARP1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PARP1 (PARP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIST1H1E_WT

Plasmid

#81762

Purpose

Gateway Donor vector containing HIST1H1E , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIST1H1E (H1-4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-FFSS-Cyclin D1

Plasmid

#215151

Purpose

Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

FFSS (FLAG-FLAG-STREP-STREP)

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAK beta

Plasmid

#141195

Purpose

Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 isoform 2 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)

Use

Human homologous recombination donor

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1beta

Plasmid

#181901

Purpose

Fluorescently tagged HP1beta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1beta (Cbx1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAKalpha_K45A

Plasmid

#141194

Purpose

Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

Changed Lysine 45 to Alanine

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

Plasmid

#141198

Purpose

Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 (MAP3K20 Human)

Tags

Strep, HA

Expression

Mammalian

Mutation

deletion of last 27 amino acids and amino acids 6…

Promoter

CMV

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

Plasmid

#228902

Purpose

For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

Depositor

Bryan Roth

Article

Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

Has Service

AAV8

Insert

hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)

Use

AAV and Synthetic Biology

Tags

HA and P2A mCitrine

Expression

Mammalian

Mutation

R108K

Promoter

Chicken Beta Actin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-p21-HA

Plasmid

#215110

Purpose

Expresses p21-HA in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV (Tet-inducible)

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAF1_WT

Plasmid

#82317

Purpose

Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAF1 (RAF1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF

Plasmid

#116719

Purpose

Lentiviral expression of BRAF

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

V600E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KIAA1549_BRAF

Plasmid

#205838

Purpose

Express mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KIAA1549_BRAF (KIAA1549, BRAF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-OGG1

Plasmid

#215135

Purpose

Expresses mCherry-OGG1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

OGG1 (OGG1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-APEX1

Plasmid

#215126

Purpose

Expresses mCherry-APEX1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

APEX1 (APEX1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV uN2C GFP (Ctl)

Plasmid

#224355

Purpose

Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

Depositor

Nicolas Charlet-Berguerand

Article

Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

Insert

uN2C

Use

AAV

Tags

eGFP

Expression

Mammalian

Mutation

Codon-optimized for expression in human, so no pu…

Promoter

CMV + chimeric intyron (CAG)

Available Since

Oct. 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-FEN1

Plasmid

#215129

Purpose

Expresses mCherry-FEN1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FEN1 (FEN1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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