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pJMC-L2-Reverse-Donor-Esp3I

Plasmid

#197844

Purpose

Donor vector to restore L2 destination via Esp3I (reverse orientation)

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro

Plasmid

#167899

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2

Plasmid

#167897

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro

Plasmid

#167903

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pAAV-VTKS3-TeTLc-P2A-mCherry

Plasmid

#178708

Purpose

AAV vector for Flp-dependent transgene expression of TeTLc-P2A-mCherry in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Publication

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

TeTLc-P2A-mCherry

Use

AAV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0090

Plasmid

#172788

Purpose

Level 0 module containing NT2_Start

Depositor

Benjamin Field

Publication

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with start codon.

Use

Synthetic Biology

Mutation

no

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto

Plasmid

#167896

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPD098 ZF43i

Plasmid

#138780

Purpose

Constitutive expression of ZF43i under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43i

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMM0494

Plasmid

#128981

Purpose

LEU2 preassembled entry vector

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

LEU2 5' homology-ConLS-sfGFP dropout-ConR1-LEU2 3' homology

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0317

Plasmid

#128979

Purpose

Encodes Zif268DBD-CRY2 under pscADH1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0320

Plasmid

#128980

Purpose

Encodes Zif268DBD-CRY2PHR under pscADH1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pscADH1-SV40NLS-ZIF268DBD-CRY2PHR (L3)-tscADH1 TRP1

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0290

Plasmid

#128978

Purpose

Reporter plasmid, pZF(3BSopp)-yEVENUS

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BSop)-yEVENUS

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0288

Plasmid

#128976

Purpose

Reporter plasmid, pZF(3BSc)-yEVENUS

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BSc)-yEVENUS

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0287

Plasmid

#128975

Purpose

Reporter plasmid, pZF(3BS)-yEVENUS

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)-yEVENUS

Expression

Yeast

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0286

Plasmid

#128974

Purpose

Reporter plasmid, pZF(3oBS)-yEVENUS

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3oBS)-yEVENUS

Expression

Yeast

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0289

Plasmid

#128977

Purpose

Reporter plasmid, pZF(4BSc)-yEVENUS

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(4BSc)-yEVENUS

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0620

Plasmid

#128995

Purpose

pTEF1-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pTEF1-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0628

Plasmid

#129003

Purpose

pRNR2-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRNR2-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0285

Plasmid

#128973

Purpose

Reporter plasmid, pZF(1BS)-yEVENUS

Depositor

Megan McClean

Publication

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(1BS)-yEVENUS

Expression

Yeast

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFH87

Plasmid

#128200

Purpose

sgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for StCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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