We narrowed to 10,326 results for: Ada;

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pX330-CANX

Plasmid

#227279

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CANX (CANX Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGPTVII-nlsSED1

Plasmid

#178190

Purpose

SED1 biosensor for expression in Arabidopsis thaliana

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

SV40 NLS

Expression

Plant

Promoter

pUBQ10

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM2_C277S-Flag

Plasmid

#180412

Purpose

lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2_C277S (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C277S

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET

Plasmid

#72542

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa)

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLA1_2

Plasmid

#160790

Purpose

Suppress POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLA1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLA1_1

Plasmid

#160789

Purpose

Suppress POLA1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLA1_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pWPI-N3ICD

Plasmid

#185524

Purpose

Constitutive overexpression of human NOTCH3 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH3 intracellular domain (NOTCH3 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b_Pa-PiuA

Plasmid

#128955

Purpose

expresses Pseudomonas aeruginosa PiuA in E. coli

Depositor

James Naismith

Article

Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

Insert

iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)

Tags

6xHis and pelB leader

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-VIM

Plasmid

#227301

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of VIM (VIM Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti 4.1 Ex miR200c-141

Plasmid

#35534

Purpose

3rd generation lentiviral vector

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

miR200c-141 genomic cluster (MIR200C, MIR141 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2012

Availability

Academic Institutions and Nonprofits only

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pET20b_Pa-PirA

Plasmid

#128956

Purpose

expresses Pseudomonas aeruginosa PirA in E. coli

Depositor

James Naismith

Article

Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

Insert

ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)

Tags

6xHis and pelB leader

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

T7

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA AXL

Plasmid

#105932

Purpose

Mammalian expression of myc-His tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Tags

His and myc

Expression

Mammalian

Promoter

tet-inducible CMV

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-F656W

Plasmid

#72546

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F656W

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-I654Q

Plasmid

#72545

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I654Q

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-L630W

Plasmid

#72543

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L630W

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBItetDT480GFP

Plasmid

#96905

Purpose

tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15

Depositor

Thomas Cooper

Article

Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)

Use

Tetracycline responsive and bidirectional

Expression

Mammalian

Promoter

tetracycline responsive and bidirectional

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1462_SERPINA1_CCA32_Caspase

Plasmid

#193194

Purpose

Express Caspase conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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p2R3a-TagRFP-OcsT

Plasmid

#71270

Purpose

Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

TagRFP

Use

Gateway

Tags

4xGly linker and octaline synthase terminator

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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