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mCherry-Climp63(1–437)

Plasmid

#186958

Purpose

Expresses mCherry-Climp63 with partial luminal domain

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Ckap4 (Ckap4 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only remain partial luminal domain

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-NS4B

Plasmid

#178846

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS4B

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-NS3

Plasmid

#178844

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-NS3

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV4-ZIKV-2KNS4B

Plasmid

#178847

Purpose

Mammalian expression of ZIKV ORF

Depositor

Judy Lieberman

Article

Sen Santara et al Proc Natl Acad Sci U S A. 2021 Nov 23;11

Insert

ZIKV-2KNS4B

Expression

Mammalian

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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SNX9

Plasmid

#171943

Purpose

Lentiviral plasmid to express mouse SNX9 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

SNX9

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1899)

Plasmid

#170143

Purpose

Expresses residues 1-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1900-2997

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-1899)

Plasmid

#170145

Purpose

Expresses residues 787-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-2138)

Plasmid

#170146

Purpose

Expresses residues 787-2138 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1547)

Plasmid

#170144

Purpose

Expresses residues 1-1547 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1548-2997

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (936-2997)

Plasmid

#170147

Purpose

Expresses residues 936-2997 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-935

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (K999R)

Plasmid

#170142

Purpose

Expresses CHD7 (K999R) in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Mutation

K999R

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA13b_gb76_GF31-2-hom_lenti

Plasmid

#138310

Purpose

Lenti expression of AcrIIA13b

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA13b

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA15_gb79_Cand27_lenti

Plasmid

#138313

Purpose

Lenti expression of AcrIIA15

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA15

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14_gb77_Cand9_lenti

Plasmid

#138311

Purpose

Lenti expression of AcrIIA14

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBS_24x-601_MA1+MA3

Plasmid

#114362

Purpose

24x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

24X601-MA1_2

Use

Unspecified

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_12x-601_MA3

Plasmid

#114360

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA3

Use

Unspecified

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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MCV-HF LTS220A/S239A

Plasmid

#112191

Purpose

Merkel cell polyomavirus molecular clone

Depositor

Patrick Moore

Article

Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

Insert

MCV-HF LTS220A/S239A

Mutation

T to G mutations at 3313 (nt) and 3370 (nt) of fu…

Promoter

None from vector. Insert contains native promoter…

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Tags

His6-tev

Expression

Insect

Mutation

K70A

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN (delta)N (aa1-117 deleted)

Plasmid

#73382

Purpose

Expresses myc tagged XPLN in mammalian cells with deletion of N-terminal amino acids 1-117

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Deleted N-terminal 1-117 aa from XPLN protein

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-GST-mEKLF

Plasmid

#67836

Purpose

Encodes GST fusion to murine EKLF (nucleotides 71-1215)

Depositor

James Bieker

Article

Zhang et al Proc Natl Acad Sci U S A. 1998 Aug 18;95

Insert

EKLF (Klf1 Mouse)

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only