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pQacR-Q1

Plasmid

#74689

Purpose

QacR-Q1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ1-QacR-PQacR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Expression

Yeast

Promoter

PGK

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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XNOR-gate-V2.0

Plasmid

#44454

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

XNOR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pHlyIIR-H1

Plasmid

#74681

Purpose

HlyIIR-H1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSH1-HlyIIR-PHlyIIR-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pYOY178

Plasmid

#23108

Depositor

Linda Wordeman

Article

Andrews et al Dev Cell. 2004 Feb . 6(2):253-68.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Mutation

S92A, S106A, S108A, S112A, S186A

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pAmtR-A1

Plasmid

#74676

Purpose

AmtR-A1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSA1-AmtR-PAmtR-YFP

Expression

Bacterial

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b-Ava3855

Plasmid

#45743

Depositor

Emily Balskus, Christopher T Walsh

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3855 (Ava_3855 Anabaena variabilis)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pLitR-L1

Plasmid

#74683

Purpose

LitR-L1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSL1-LitR-PLitR-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-S3

Plasmid

#74693

Purpose

SrpR-S3 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS3-SrpR-PSrpR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAmeR-F1

Plasmid

#74675

Purpose

AmeR-F1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSF1-AmeR-PAmeR-YFP

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLPhygCAS9

Plasmid

#63555

Purpose

Expresses CAS9 in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)

Use

Leishmania expression

Tags

flag

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pSrpR-S1

Plasmid

#74691

Purpose

SrpR-S1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSS1-SrpR-PSrpR-YFP

Expression

Bacterial

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pIcaRA-I1

Plasmid

#74682

Purpose

IcaRA-I1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSI1-IcaRA-PIcaR-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAD

Plasmid

#64747

Purpose

Yeast expression plasmid: ACC1 (ser659ala, ser1157ala)

Depositor

Jens Nielsen

Article

Shi et al MBio. 2014 May 6;5(3):e01130-14. doi: 10

Insert

ACC1 (ser659ala, ser1157ala)

Expression

Yeast

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLmrA-N1

Plasmid

#74684

Purpose

LmrA-N1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSN1-LmrA-PLmrA-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/mNeptune2-nat

Plasmid

#120337

Purpose

Bacterial mNeptune2 labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Expression

Bacterial

Promoter

proD

Available Since

Aug. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD3-mClover-nat

Plasmid

#120328

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Expression

Bacterial

Mutation

D103N (see comments below)

Promoter

(None)

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHH04_32_sRNA-A_Sp_coD

Plasmid

#120900

Purpose

the component plasmid of a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

pECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7xTS

Plasmid

#117389

Purpose

Tn7 tagging vector with temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pQMD

Plasmid

#48103

Purpose

Destination plasmid (Gateway cloning) for cumate-inducible expression of myc-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

myc tag

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 28, 2014

Availability

Academic Institutions and Nonprofits only

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