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We narrowed to 11,277 results for: loxP

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Showing: 5521 - 5540 of 11277 results

  4. pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR

    Plasmid
    #196219
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFR
    Depositor
    Kaspar Podgorski
    Publication
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has service
    AAV2
    Insert
    pAAV CAG FLEX iGluSnFR3 v857.PDGFR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and PDGFR TM Domain
    Expression
    Mammalian
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196219-plasmid-map-sequence-id-405480

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  7. pPL001-BBTK

    Plasmid
    #184751
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.
    Depositor
    Jef Boeke
    Publication
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    184751-plasmid-map-sequence-id-365380

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  8. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

    Plasmid
    #189630
    Purpose
    Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.
    Depositor
    Jerzy Szablowski
    Publication
    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Inserts
    Gaussia luciferase fused to Fc
    IRES-EGFP
    Use
    AAV, Cre/Lox, and Luciferase
    Tags
    Gluc and IgG1 Fc
    Expression
    Mammalian
    Promoter
    IRES and hSyn
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    189630-plasmid-map-sequence-id-376528

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  10. pAAV-flex-GFP-shRNA-GluN1

    Plasmid
    #182502
    Purpose
    Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter
    Depositor
    William Wisden
    Publication
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Expression
    Mammalian
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182502-plasmid-map-sequence-id-355962

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  11. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Publication
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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  13. mVenus-hCOL1A1

    Plasmid
    #140111
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140111-plasmid-map-sequence-id-296751

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  14. pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP

    Plasmid
    #135176
    Purpose
    AAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.
    Depositor
    Tetsuo Yamamori
    Publication
    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116
    Inserts
    hChR2
    TRE3G
    Use
    AAV and Cre/Lox
    Tags
    EYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    E123T and T159C
    Promoter
    TRE3G
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    135176-plasmid-map-sequence-id-268585

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  16. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105983
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Publication
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    SYFP2-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Promoter
    human Synapsin 1
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105983-plasmid-map-sequence-id-235283

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  17. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Publication
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

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  18. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  20. pAav-TP53-T2A-BirA*

    Plasmid
    #115652
    Purpose
    rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette
    Depositor
    Sven Eyckerman
    Publication
    Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102
    Inserts
    TP53 (TP53 Human)
    TP53 (TP53 Human)
    T2A
    BirA*
    Use
    AAV
    Tags
    BirA* and T2A
    Mutation
    Homology region 1 and Homology region 2
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    115652-plasmid-map-sequence-id-223051

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Showing: 5521 - 5540 of 11277 results