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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,509 results for: nls

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Showing: 5541 - 5560 of 6509 results

  1. pYPQAT06

    Plasmid
    #223378
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223378-plasmid-map-sequence-id-454174

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  2. pCAT079

    Plasmid
    #180509
    Purpose
    Plasmid expressing mammalian codon optimized wt DpbCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available Since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180509-plasmid-map-sequence-id-364076

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  3. dpYPQ230-RRV-CBE

    Plasmid
    #250717
    Purpose
    dLbCas12a-RRV based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-RRV with the linker of (GGGGS)x6
    Depositor
    Yiping Qi
    Article
    Cheng et al J Integr Plant Biol. 2026 Apr 2. doi: 10
    Insert
    hA3A/Y130F-(GGGGS)x6-dLbCas12a-RRV-UGI
    Use
    CRISPR
    Tags
    5' and 3' NLS
    Expression
    Plant
    Available Since
    June 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250717-plasmid-map-sequence-id-512023

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  5. dCas9-ryt

    Plasmid
    #246022
    Purpose
    Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex
    Depositor
    Max Scott
    Inserts
    dCas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    246022-plasmid-map-sequence-id-492666

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  6. dCas9-tra135

    Plasmid
    #246023
    Purpose
    Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    dCas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    246023-plasmid-map-sequence-id-492668

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  7. dCas9-tra246

    Plasmid
    #246024
    Purpose
    Expresses dCas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    dCas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    246024-plasmid-map-sequence-id-492667

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  16. pGGG-TaBSS1-tdTom

    Plasmid
    #242014
    Purpose
    L2 Golden Gate construct for in planta (wheat) expression of transgenic tdTomato driven by the Basal Spikelet Specific 1 regulatory environment.
    Depositor
    Cristobal Uauy
    Insert
    tdTomato
    Tags
    NLS
    Expression
    Plant
    Promoter
    BSS1
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242014-plasmid-map-sequence-id-487879

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  17. pYPQAT52

    Plasmid
    #223424
    Purpose
    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223424-plasmid-map-sequence-id-454179

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  18. pYPQAT53

    Plasmid
    #223425
    Purpose
    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223425-plasmid-map-sequence-id-454170

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  19. pYPQAT54

    Plasmid
    #223426
    Purpose
    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-ecTadA8e-dLbCas12a-D156R-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223426-plasmid-map-sequence-id-452223

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  20. pYPQAT34

    Plasmid
    #223406
    Purpose
    T-DNA vector for SpCas9-D10A based A-to-G base editing for monocot plants; NGG PAM; SpCas9D10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpCas9-D10A-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223406-plasmid-map-sequence-id-454165

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Showing: 5541 - 5560 of 6509 results