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pGS-BacA-21222-SNF5

Plasmid

#109186

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged SNF5 under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Insert

SNF5 (SMARCB1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

Plasmid

#240228

Purpose

Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-sfGFP-TurboID-ER

Plasmid

#240232

Purpose

Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-CG6867-sfGFP

Plasmid

#240237

Purpose

Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

CG6867 (CG6867 Fly)

Tags

sfGFP

Expression

Insect

Promoter

UAS

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_spatzle-HA

Plasmid

#240227

Purpose

Gateway entry clone with spatzle tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

spatzle (spz Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hBIRC3_1k)-PGKpuro2ABFP-W

Plasmid

#208418

Purpose

Lentiviral gRNA plasmid targeting human BIRC3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

BIRC3 (BIRC3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hBIRC3_2b)-PGKpuro2ABFP-W

Plasmid

#208419

Purpose

Lentiviral gRNA plasmid targeting human BIRC3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

BIRC3 (BIRC3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hCASP8_1k)-PGKpuro2ABFP-W

Plasmid

#208422

Purpose

Lentiviral gRNA plasmid targeting human CASP8 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

CASP8 (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hCASP8_2b)-PGKpuro2ABFP-W

Plasmid

#208423

Purpose

Lentiviral gRNA plasmid targeting human CASP8 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

CASP8 (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_1k)-PGKpuro2ABFP-W

Plasmid

#208424

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_2b)-PGKpuro2ABFP-W

Plasmid

#208425

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hTNF_2b)-PGKpuro2ABFP-W

Plasmid

#208426

Purpose

Lentiviral gRNA plasmid targeting human TNF gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

TNF (TNF Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMAP3K7_1k)-PGKpuro2ABFP-W

Plasmid

#208412

Purpose

Lentiviral gRNA plasmid targeting human MAP3K7 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

MAP3K7 (MAP3K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMAP3K7_2m)-PGKpuro2ABFP-W

Plasmid

#208413

Purpose

Lentiviral gRNA plasmid targeting human MAP3K7 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

MAP3K7 (MAP3K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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V2R untagged

Plasmid

#208911

Purpose

encodes the human vasopressin receptor 2, without any tags

Depositor

Christian Gruber

Article

Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

Insert

AVPR2 (AVPR2 Human)

Expression

Mammalian

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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V1bR untagged

Plasmid

#208925

Purpose

encodes the human vasopressin receptor 1b, without any tags

Depositor

Christian Gruber

Article

Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

Insert

AVPR1B (AVPR1B Human)

Expression

Mammalian

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pOP795

Plasmid

#235700

Purpose

Protein expression His-taged HsNRBF2_FL in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 (NRBF2 Human)

Tags

His6(TEV)

Expression

Bacterial

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pOP796

Plasmid

#235701

Purpose

Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 MIT domain (NRBF2 Human)

Tags

His6(TEV)

Expression

Bacterial

Mutation

aa 1-89

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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